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Planta Med 2020; 86(03): 198-204
DOI: 10.1055/a-1078-7860
Biological and Pharmacological Activity
Original Papers
Georg Thieme Verlag KG Stuttgart · New York

Suppressive Effects of Hot-water Extract of Magnolia obovata on Clostridium perfringens Enterotoxin-induced Cytotoxicity in Human Intestinal Caco-2 Cells

Takeshi Kawahara
1   Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
,
Kotomi Fujii
1   Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
,
Kouki Nakajima
1   Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
,
Rikuri Fujii
1   Academic Assembly School of Science and Technology, Institute of Agriculture, Shinshu University
,
Shuichiro Inagaki
2   Department of Health and Nutrition, Faculty of Health and Nutrition, Osaka Shoin Womenʼs University
,
Kunio Hara
3   Kiso Town Resources Institute
,
Hisako Yasui
3   Kiso Town Resources Institute
› Author Affiliations
Further Information

Publication History

received 15 July 2019
revised 02 December 2019

accepted 06 December 2019

Publication Date:
02 January 2020 (online)

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Abstract

The physiological functions of the leaves of Japanese big-leaf magnolia (Magnolia obovata) against enterotoxins produced by foodborne pathogens remain unclear. In this study, we evaluated the protective effects of M. obovata leaf extract (MLE) against the cytotoxicity of Clostridium perfringens enterotoxin (CPE), which causes the symptoms of C. perfringens type A food poisoning. The protective effects of MLE against CPE-induced cytotoxicity were evaluated in human intestinal epithelial Caco-2 cells. Pre-treatment with MLE significantly suppressed the cytotoxicity induced by CPE in undifferentiated and differentiated human intestinal Caco-2 cells at a pH range of 4.0 – 7.0. This CPE-suppressive effect was due to a hydrophilic sugar-containing compound without phenolic and protein structures but not the hydrophobic biologically active neolignans, honokiol and magnolol. MLE had a protective effect against cytotoxicity caused by type A C. perfringens. Our results provide novel insight regarding the usage of M. obovata in managing food poisoning.

Key words

Magnolia obovata - Magnoliaceae - Clostridium perfringens - enterotoxin - food poisoning - claudin

Supporting Information

    The effect of magnoloside A on CPE-induced cytotoxicity in Caco-2 cells is available as supplemental data.

  • Supporting Information
 

  • References

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