Trichothecenes from an Endophytic Fungus Alternaria sp. sb23

Ying Gao; Jia Zhou; Hanli Ruan

doi:10.1055/a-1091-8831

Planta Medica, Inhaltsverzeichnis

Planta Med 2020; 86(13/14): 976-982
DOI: 10.1055/a-1091-8831

Biological and Pharmacological Activity

Original Papers

Trichothecenes from an Endophytic Fungus Alternaria sp. sb23

Ying Gao

School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China

,

Jia Zhou

School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China

,

Hanli Ruan

School of Pharmacy, Tongji Medical College of Huazhong University of Science and Technology, Hubei Key Laboratory of Natural Medicinal Chemistry and Resource Evaluation, Wuhan, Peopleʼs Republic of China

› Institutsangaben

Abstract

Three new (alterchothecenes A – C, 1 –3) and 3 known (4 –6) trichothecenes, along with 9 known compounds (7 –15), were isolated from the culture of Alternaria sp. sb23, an endophytic fungus separated from the root of Schisandra sphenanthera Rehd. et Wils. Their structures were elucidated by spectroscopic analyses, and the absolute configurations of 1–3 were determined through comparison of the experimental electronic circular dichroism (ECD) spectra and optical rotations with similar analogues. In vitro cytotoxicity tests of compounds 1–6 against human HT-29 colon carcinoma and human MCF-7 breast cancer cell lines indicated that 4–6 exhibited significant cytotoxic effects, with IC₅₀ values ranging from 0.89 to 9.38 µM. And the potential of compounds 1–6 as tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) sensitizers in HT-29 cells was evaluated. The results revealed that combination treatment of TRAIL with compounds 1–6 synergistically decreased cell viability compared with the sole treatment with those compounds.

Key words

Alternaria sp. - endophytic fungus - trichothecenes - cytotoxic effects - TRAIL sensitizer

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Referenzen

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