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Planta Med 2020; 86(13/14): 983-987
DOI: 10.1055/a-1102-1168
Biological and Pharmacological Activity
Original Papers

Mogroside V-producing Endophytic Fungi Isolated from Siraitia grosvenorii

Chen Bin
1   Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China
2   College of Environment and Resource, Guangxi Normal University, Guilin, China
,
Yu Fangming
1   Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China
2   College of Environment and Resource, Guangxi Normal University, Guilin, China
,
Jiang Zhi
1   Key Laboratory of Ecology of Rare and Endangered Species and Environmental Protection, Guangxi Normal University, Ministry of Education, Guilin, China
2   College of Environment and Resource, Guangxi Normal University, Guilin, China
› InstitutsangabenGefördert durch: National Natural Science Foundation of China 41661077
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Abstract

Endophytic fungi can produce beneficial active components during symbiosis with host plants. Fifteen purified endophytic fungal strains were isolated and screened from root, stem, leaf, and fruit of the Chinese medicine food homologous plant Siraitia grosvenorii. These strains were fermented, and then the fermented solution was extracted using acetonitrile. The extracts were analyzed by high performance liquid chromatography (HPLC) and mass spectrum (MS), using standard mogroside V as control. The results showed that strain LHG-F5 and strain LHG-L4 could produce mogroside V with output of 3.814 µg/mL and 2.675 µg/mL, respectively. By morphological observation together with similarity of internal transcribed spacer (ITS) sequence by Clustan X method, LHG-F5 and LHG-L4 were classified as Diaporthe angelica and Fusarium solani, respectively.

Key words

Siraitia grosvenorii - Cucurbitaceae - endophytic fungi - mogroside V - HPLC - LC-MS


Publikationsverlauf

Eingereicht: 01. Juli 2019

Angenommen nach Revision: 18. Januar 2020

Artikel online veröffentlicht:
31. Januar 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Stuttgart · New York

 

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