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CC BY-NC-ND 4.0 · Planta Medica International Open 2020; 7(01): e17-e25
DOI: 10.1055/a-1111-9907
Original Papers
Eigentümer und Copyright ©Georg Thieme Verlag KG 2020

An Ethanolic Extract of Boehmeria caudata Aerial Parts Displays Anti-inflammatory and Anti-tumor Activities

Paula Pereira de Paiva 
1   Institute of Biology, University of Campinas, Campinas, Brazil
2   Chemical, Biological and Agricultural Research Center, (CPQBA), University of Campinas, Paulínia, Brazil
3   Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, Brazil
,
Fabiana Regina Nonato
2   Chemical, Biological and Agricultural Research Center, (CPQBA), University of Campinas, Paulínia, Brazil
,
Ana Lúcia Tasca Gois Ruiz 
3   Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, Brazil
,
Ilza Maria de Oliveira Sousa
2   Chemical, Biological and Agricultural Research Center, (CPQBA), University of Campinas, Paulínia, Brazil
3   Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, Brazil
,
Rafael Rosolen Teixeira Zafred
1   Institute of Biology, University of Campinas, Campinas, Brazil
2   Chemical, Biological and Agricultural Research Center, (CPQBA), University of Campinas, Paulínia, Brazil
,
Diogo Noin de Oliveira
3   Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, Brazil
,
Rodrigo Ramos Catharino
3   Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, Brazil
,
Mary Ann Foglio
3   Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, Brazil
,
João Ernesto de Carvalho
3   Faculty of Pharmaceutical Sciences, University of Campinas, Campinas, Brazil
› Institutsangaben
Weitere Informationen

Publikationsverlauf

received 20. November 2019
revised 18. Januar 2020

accepted 22. Januar 2020

Publikationsdatum:
18. März 2020 (online)

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Abstract

The tumor microenvironment presents several therapeutic targets, with inflammation being one of them. In search of new drugs, plants have shown to be an effective source of potent anti-inflammatory and anticancer agents. This study aimed to evaluate the antitumoral and inflammatory activities of Boehmeria caudata aerial parts extract. Bioguided in vitro antiproliferative screening showed that phenanthroquinolizidine obtained from the aerial B. caudata ethanolic extract had a straight relationship with activity. Moreover, the orally administered ethanolic extract reduced Ehrlich solid tumor growth and displayed an anti-inflammatory effect in both evaluated experimental models (carrageenan-induced paw edema and croton oil-induced ear edema). These results suggest that the antitumor activity of the ethanolic extract could be explained by antiproliferative effects associated with anti-inflammatory action.

Key words

Boehmeria caudate - Urticaceae - inflammation - antineoplastic agents - Ehrlich tumor carcinoma - drug screening assays

Supporting information

 

  • References

  • 1 Diakos CI, Charles KA, McMillan DC, Stephen JClarke. Cancer-related inflammation and treatment effectiveness. Lancet Oncol 2014; 15: e493-e503
    CrossrefPubMedGoogle Scholar
  • 2 Abdul Wahab SM, Jantan I, Haque MA, Laiba Arshad. Exploring the leaves of Annona muricata L. as a source of potential anti-inflammatory and anticancer agents. Front Pharmacol 2018; 9: 661
    CrossrefPubMedGoogle Scholar
  • 3 Ahmad W, Jantan I, SNA Bukhari. Tinospora crispa (L.) Hook. f. & Thomson: A Review of Its Ethnobotanical, Phytochemical, and Pharmacological Aspects. Front Pharmacol 2016; 7: 59
    PubMedGoogle Scholar
  • 4 Ma JF, Wei PF, Guo C, Shi YP, Qiu LX, Wen LP. The Ethyl Acetate Extract of Gynura formosana Kitam. Leaves Inhibited Cervical Cancer Cell Proliferation via Induction of Autophagy. Biomed Res Int 2018; 2018: 4780612
    PubMedGoogle Scholar
  • 5 Pais VAA. Evaluation of anticancer activity in vivo and in vitro of medicinal plants from “Serra da Mantiqueira Paulista” [dissertation]. Campinas: Institute of Biology, University of Campinas; 2011
    Google Scholar
  • 6 Wilmot-Dear CM, Friis I. The New World species of Boehmeria and Pouzolzia (Urticaceae, tribus Boehmerieae). A taxonomic revision. Opera Botanica 1996; 129: 1-103
    PubMedGoogle Scholar
  • 7 Gaglioti AL. Urticaceae Juss. no Estado de São Paulo, Brasil [dissertation]. São Paulo: Instituto de Botânica da Secretaria de Estado do Meio Ambiente. 2011
    PubMedGoogle Scholar
  • 8 Hoffmann JJ, Luzbetak DJ, Torrance SJ, Cole JR. Cryptopleurine, cytotoxic agent from Boehmeria caudata (Urtiaceae) and Cryptocarya laevigata (Lauraceae). Phytochemistry 1977; 17: 1448
    PubMedGoogle Scholar
  • 9 Farnsworth NR, Hart NK, Johns SR, Lamberton JA, Messmer W. Alkaloids of Boehmeria cylindrica (family Urticaceae): Identification of a cytotoxic agent, highly active against Eagle’s 9KB carcinoma of the nasopharynx in cell culture, as crytopleurine. Aust J Chem 1969; 22: 1805-1807
    CrossrefPubMedGoogle Scholar
  • 10 Lin CC, Yen MH, Lo TS, Lin CF. The anti-inflammatory and liver protective effects of Boehmeria nivea and B. nivea subsp. nippononivea in rats. Phytomedicine 1997; 4: 301-308
    CrossrefPubMedGoogle Scholar
  • 11 Luo Y, Liu Y, Luo D, Gao X, Li B, Zang G. Cytotoxic alkaloids from Boehmeria siamensis. Planta Med 2003; 69: 842-845
    Artikel in Thieme ConnectPubMedGoogle Scholar
  • 12 Wardihan, Rusdi M, Alam G, Lukman. Manggau M. Selective cytotoxicity evaluation in anticancer drug screening of Boehmeria virgata (Forst) Guill leaves to several human cell lines: Hela, Widr, T47D and Vero. Dhaka Univ J Pharm Sci 2013; 12: 87-90
    PubMedGoogle Scholar
  • 13 Sung MJ, Davaatseren M, Kim SH, Kim MJ, Hwang J-T. Boehmeria nivea attenuates LPS induced inflammatory markers by inhibiting p38 and JNK phosphorylations in RAW264.7 macrophages. Pharm Biol 2013; 51: 1131-1136
    CrossrefPubMedGoogle Scholar
  • 14 Lee AY, Wang X, Lee DG, Kim Y-M, Jung Y-S, Kim HB, Kim HY, Cho EJ, Lee S. Various Biological Activities of Ramie (Boehmeria nivea). Appl Biol Chem 2014; 57: 279-286
    CrossrefPubMedGoogle Scholar
  • 15 Fouche G, Cragg GM, Pillay P, Kolesnikova N, Maharaj VJ, Senabe J. In vitro anticancer screening of South African plants. J Ethnopharmacol 2008; 119: 455-461
    CrossrefPubMedGoogle Scholar
  • 16 Al-Shamma A, Drake SD, Guagliardi LE, Mitscher LA, Swayze JK. Antimicrobial alkaloids from Boehmeria cylindrical. Phytochemistry 1982; 21: 485-487
    CrossrefPubMedGoogle Scholar
  • 17 Krmpotic E, Farnsworth NR, Messmer WM. Cryptopleurine, an active antiviral alkaloid from Boehmeria cylindrical (L.) Sw. (Urticaceae). J Pharm Sci 1972; 61: 1508-1509
    CrossrefPubMedGoogle Scholar
  • 18 Jin HR, Jin SZ, Cai XF, Li D, Wu X, Nan JX, Lee JJ, Jin X. Cryptopleurine targets NF-kB pathway, leading to inhibition of gene products associated with cell survival, proliferation, invasion and angiogenesis. PLoS One 2012; 7: e40355
    CrossrefPubMedGoogle Scholar
  • 19 OECD. OCDE Guidelines for the Testing of Chemicals, Acute Oral Toxicity – up-and-down procedure (UDP). Available at: https://www.oecd.org/env/test-no-425-acute-oral-toxicity-up-and-down-procedure-9789264071049-en.htm Accessed January, 25 2014.
    PubMed
  • 20 Meral I, Pala M, Akbas F, Ustunova S, Yildiz C, Demirel MH. Effects of thymoquinone on liver miRNAs and oxidative stress in Ehrlich acid mouse solid tumor model. Biotech Histochem. 2018; 93: 301-308
    CrossrefPubMedGoogle Scholar
  • 21 Iwamoto LH, Vendramini-Costa DB, Monteiro PA, Ruiz AL, Sousa IM, Foglio MA, de Carvalho JE, Rodrigues RAF. Anticancer and Anti-Inflammatory Activities of a Standardized Dichloromethane Extract from Piper umbellatum L. Leaves. Evid Based Complement Alternat Med 2015; 2015: 948737
    PubMedGoogle Scholar
  • 22 Fabri RL, Garcia RA, Florêncio JR, Pinto NCC, de Oliveira LG, Aguiar JAK, Ribeiro A, Scio E. Anti-inflammatory and antioxidative effects of the methanolic extract of the aerial parts of Mitracarpus frigidusin established animal models. J Pharm Pharmacol 2014; 66: 722-732
    CrossrefPubMedGoogle Scholar
  • 23 Lapa AJ, Souccar C, Lima-Landman T MR, Castro MAS, Lima TCM. Métodos de avaliação da atividade farmacológica de plantas medicinais, 5ª. ed. São Paulo Setor de Produtos Naturais, Departamento de Farmacologia, UNIFESP/EPM. 2007 119p.
    PubMedGoogle Scholar
  • 24 Pedernera AM, Guardia T, Calderón CEG, Rotelli AE, de la Rocha NE, Saad JR, Verrilli MA, Aseff SG, Pelzer LE. Anti-inflammatory effect of Acacia visco extracts in animal models. Inflammopharmacology 2010; 18: 253-260
    CrossrefPubMedGoogle Scholar
  • 25 Fridrichsons J. Mathieson AMCL. Structure of a derivative of cryptopleurine. Nature 1954; 173: 732-733
    CrossrefPubMedGoogle Scholar
  • 26 Almeida VL, Leitão A, Reina LCB, Montanari A, Donnici CL, Lopes MTR. Câncer e a agentes antineoplásicos ciclo-celular específicos e ciclo-celular não específico que interagem com o DNA: uma introdução. Quim Nova 2005; 28: 118-129
    CrossrefPubMedGoogle Scholar
  • 27 Brunherotti MR. Extravasation intervention of vesicant chemotherapeutic agents: Integrative literature review [dissertation]. Ribeirão Preto Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, Universidade de São Paulo. 2007
    PubMedGoogle Scholar
  • 28 Prajapati VD, Maheriya PM, Jani GK, Solanki HK. Carrageenan: A natural seaweed polysaccharide and its applications. Carbohydr Polym 2014; 105: 97-112
    CrossrefPubMedGoogle Scholar
  • 29 Murakawa M, Yamaoka K, Tanaka Y, Fukuda Y. Involvement of tumor necrosis factor (TNF)-α in phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced skin edema in mice. Biochem Pharmacol 2006; 71: 1331-1336
    CrossrefPubMedGoogle Scholar
  • 30 Tubaro A, Dri P, Melato M, Mulas G, Bianchi P, Del Negro P, Della Loggia R. Agents Actions 1996; 19: 371-373
    PubMed
  • 31 Carlson RP, O’Neill-Davis L, Chang J, Lewis AJ. Modulation of mouse ear oedema by cyclooxygenase and lipoxygenase inhibitors and other pharmacologic agents. Agents Actions 1985; 17: 197-206
    CrossrefPubMedGoogle Scholar
  • 32 Paiva P, Nonato F, Zafred R, Sousa I, Ruiz AL, Carvalho JE. Anti-inflammatory activity of Boehmeria caudata sw. (Urticaceae) crude ethanol extract on croton oil mouse ear oedema. Planta Med 2014; 80: 1528
    PubMedGoogle Scholar
  • 33 Schneider C, Pozzi A. Cyclooxygenases and lipoxygenases in cancer. Cancer Metastasis Rev 2011; 30: 277-294
    CrossrefPubMedGoogle Scholar
  • 34 Greene ER, Huang S, Serhan CN, Panigrahy D. Regulation of inflammation in cancer by eicosanoids. Prostaglandins Other Lipid Mediat 2011; 96: 27-36
    CrossrefPubMedGoogle Scholar
  • 35 Gibbons S. An introduction to planar chromatography and its application to natural products isolation. In: Sarker SD, Nahar L (Editors.) Natural Products Isolation – Methods and Protocols. 3 ed. New York: Humana Press (Springer); 2012: 117-154
    Google Scholar
  • 36 de Oliveira JF, Nonato FR, Zafred RRT, Leite NMSL, Ruiz ALTG, Carvalho JE, Silva AL, Moura RO, Lima MCA. Evaluation of anti-inflammatory effect of derivative (E)-N-(4- bromophenyl)-2-(thiophen-2-ylmethylene)-thiosemicarbazone. Biomed Pharmacother 80 2016; 388-392
    CrossrefPubMedGoogle Scholar