Daucosterol linolenate from Sweet Potato Suppresses MCF7-Xenograft-Tumor Growth through Regulating PI3K/AKT Pathway

Bing Han; Lingmin Jiang; Pu Jiang; Deqi Zhou; Xiaoxin Jia; Xuegang Li; Xiaoli Ye

doi:10.1055/a-1176-1884

Planta Medica, Table of Contents

Planta Med 2020; 86(11): 767-775
DOI: 10.1055/a-1176-1884

Biological and Pharmacological Activity

Original Papers

Georg Thieme Verlag KG Stuttgart · New York

Daucosterol linolenate from Sweet Potato Suppresses MCF7-Xenograft-Tumor Growth through Regulating PI3K/AKT Pathway

Bing Han‡

¹Chongqing Productivity Promotion Center for the Modernization of Chinese Traditional Medicine, School of Pharmaceutical Sciences, Southwest University, Chongqing, China

³Laboratory of Medicinal Plant Biotechnology, College of Pharmacy, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China

,

Lingmin Jiang‡

¹Chongqing Productivity Promotion Center for the Modernization of Chinese Traditional Medicine, School of Pharmaceutical Sciences, Southwest University, Chongqing, China

,

Pu Jiang

¹Chongqing Productivity Promotion Center for the Modernization of Chinese Traditional Medicine, School of Pharmaceutical Sciences, Southwest University, Chongqing, China

,

Deqi Zhou

⁴Oncology Department, Chongqing Beibei District Hospital of Traditional Chinese Medicine, Chongqing, China

,

Xiaoxin Jia

⁴Oncology Department, Chongqing Beibei District Hospital of Traditional Chinese Medicine, Chongqing, China

,

Xuegang Li

¹Chongqing Productivity Promotion Center for the Modernization of Chinese Traditional Medicine, School of Pharmaceutical Sciences, Southwest University, Chongqing, China

,

Xiaoli Ye

²Chongqing Engineering Research Centre for Sweet Potato, School of Life Sciences, Southwest University, Chongqing, China

› Author Affiliations

Abstract

Sweet potato is a functional food with potential antitumor properties, but the bioactive constituents and biological mechanisms remain unclear. In this study, we investigated the antitumor effect of daucosterol linolenate extracted from sweet potato and its potential mechanism. An MTT assay indicated that DLA inhibited the proliferation of breast cancer MCF-7 cells but had only weak effects on the proliferation of MDA-MB-231, 4T1, and MCF-10A cells. Flow cytometry analysis revealed that daucosterol linolenate induced apoptosis of MCF-7 cells. Experiments with MCF-7 xenograft in nude mice further confirmed that DLA inhibited tumor growth dose-dependently. After DLA treatment, the expressions of B-cell lymphoma 2 and vascular endothelial growth factor were decreased and that of cleaved caspase 3 was increased as compared to the TC group. DLA also down-regulated the expression of phosphoinositide 3-kinase/protein kinase B and repressed insulin-induced phosphoinositide 3-kinase/protein kinase B activation. Our findings suggest that DLA suppresses breast tumor growth through inactivating the phosphoinositide 3-kinase/protein kinase B pathway.

Key words

Ipomoea batatas - Convolvulaceae - Daucosterol linolenate - breast cancer - phosphoinositide 3-kinase/protein kinase B

Full Text

References

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