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DOI: 10.1055/a-1197-4293
Mütter-Screening in einem COVID-19-Niedrig-Pandemiegebiet: Bestimmung SARS-CoV-2-spezifischer Antikörper bei 401 Rostocker Müttern mittels ELISA und Immunfluoreszenz-Bestätigungstest
Screening of Mothers in a COVID-19 Low-Prevalence Region: Determination of SARS-CoV-2 Antibodies in 401 Mothers from Rostock by ELISA and Confirmation by Immunofluorescence
Zusammenfassung
Hintergrund Bei Kindern verläuft die Infektion mit SARS-CoV-2, dem Erreger von COVID-19, in der Regel weniger schwer und häufiger symptomlos als bei Erwachsenen. Kinder können aber diese Infektion u. a. auf vulnerable Erwachsene übertragen. Daher sind zur Zeit Einrichtungen der Kinderbetreuung und Schulen teilweise geschlossen.
Methoden Da sich der Infektionsstatus bei Kindern unter 13 Jahren und deren Müttern meist ähnlich darstellt, untersuchten wir bei 401 Rostocker Müttern von Kindern zwischen 1 und 10 Jahren Rachenabstriche auf SARS-CoV-2 und Serumproben auf Antikörper gegen diesen Erreger.
Ergebnisse Wir fanden bei keiner der untersuchten Mütter RNA von SARS-CoV-2 im Rachenabstrich. Im ELISA waren IgG-Antikörper in einem Serum positiv, IgA-Antikörper waren in 11 Seren positiv und in 3 Seren grenzwertig. Alle 401 Seren waren im Fluoreszenz-Bestätigungstest (IIFT) mit FITC-markierten IgG-, IgA- und IgM-Antikörpern negativ.
Schlussfolgerung Im Untersuchungszeitraum waren bei den getesteten Rostocker Müttern weder RNA von SARS-CoV-2 noch spezifische Antikörper nachweisbar.
Abstract
Background In children, the infection with SARS-CoV-2, the cause of COVID-19, tends to be clinically inapparent more often or less severe than in adults. The spread of this infection from children poses a danger to vulnerable adults. Therefore, child care institutions and schools currently are widely closed.
Methods Since the status of infection tends to be congruent in mothers and their children, we tested 401 mothers of children between 1 and 10 years in the city of Rostock (State of Mecklenburg-Westpomerania, northeast of Germany), for the presence of RNA of SARS-CoV-2 in throat swabs, and of antibodies against SARS-CoV-2 in serum.
Results In none of the mothers tested, RNA of this virus was detected in the throat swab. In the ELISA test, IgG antibodies were positive in one serum sample, IgA antibodies were positive in 11, and borderline in 3 serum samples. All 401 sera were negative in the indirect immunofluorescence test (IIFT) with FITC-labeled IgG, IgA, und IgM antibodies.
Conclusion At the time of this study, neither SARS-CoV-2 RNA, nor specific antibodies against SARS-CoV-2 were detectable in the mothers tested in Rostock.
Publication History
Article published online:
22 June 2020
© .
© Georg Thieme Verlag KG
Stuttgart · New York
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