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Planta Med 2020; 86(18): 1345-1352
DOI: 10.1055/a-1214-6718
Biological and Pharmacological Activity
Original Papers

Codonopsis lanceolata Contributes to Ca2+ Homeostasis by Mediating SOCE and PLC/IP3 Pathways in Vascular Endothelial and Smooth Muscle Cells

Min Kyung Kim‡
1   Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
,
A Young Han‡
1   Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
,
You Kyoung Shin
1   Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
,
Kwang-Won Lee
2   Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul, Republic of Korea
,
Geun Hee Seol 
1   Department of Basic Nursing Science, College of Nursing, Korea University, Seoul, Republic of Korea
› Author AffiliationsSupported by: Basic Science Research Program through the National Research Foundation of Korea NRF-2018R1D1A1B07050048
Supported by: National Research Foundation of Korea Forest Service KFS-2016005C10-1719-AB01
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Abstract

Codonopsis lanceolata has been widely used as an anti-inflammatory and anti-lipogenic agent in traditional medicine. Recently, C. lanceolata was reported to prevent hypertension by improving vascular function. This study evaluated the effects of C. lanceolata and its major component lancemaside A on cytosolic calcium concentration in vascular endothelial cells and vascular smooth muscle cells. Cytosolic calcium concentration was measured using fura-2 AM fluorescence. C. lanceolata or lancemaside A increased the cytosolic calcium concentration by releasing Ca2+ from the endoplasmic reticulum and sarcoplasmic reticulum and by Ca2+ entry into endothelial cells and vascular smooth muscle cells from extracellular sources. The C. lanceolata- and lancemaside A-induced cytosolic calcium concentration increases were significantly inhibited by lanthanum, an inhibitor of non-selective cation channels, in both endothelial cells and vascular smooth muscle cells. Moreover, C. lanceolata and lancemaside A significantly inhibited store-operated Ca2+ entry under pathological extracellular Ca2+ levels. In Ca2+-free extracellular fluid, increases in the cytosolic calcium concentration induced by C. lanceolata or lancemaside A were significantly inhibited by U73122, an inhibitor of phospholipase C, and 2-APB, an inositol 1,4,5-trisphosphate receptor antagonist. In addition, dantrolene treatment, which inhibits Ca2+ release through ryanodine receptor channels, also inhibited C. lanceolata- or lancemaside A-induced increases in the cytosolic calcium concentration through the phospholipase C/inositol 1,4,5-trisphosphate pathway. These results suggest that C. lanceolata and lancemaside A increase the cytosolic calcium concentration through the non-selective cation channels and phospholipase C/inositol 1,4,5-trisphosphate pathways under physiological conditions and inhibit store-operated Ca2+ entry under pathological conditions in endothelial cells and vascular smooth muscle cells. C. lanceolata or lancemaside A can protect endothelial cells and vascular smooth muscle cells by maintaining cytosolic calcium concentration homeostasis, suggesting possible applications for these materials in diets for preventing vascular damage.

Key words

Codonopsis lanceolate - Campanulaceae - store-operated Ca2+ entry - inositol 1,4,5-trisphosphate - vascular endothelial cells - vascular smooth muscle cells


Publication History

Received: 06 March 2020

Accepted after revision: 01 July 2020

Article published online:
30 July 2020

© 2020. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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