Hydroalcoholic Extract of Myrcia bella Loaded into a Microemulsion System: A Study of Antifungal and Mutagenic Potential

Gabriel Davi Marena; Luiza Girotto; Luiz Leonardo Saldanha; Matheus Aparecido dos Santos Ramos; Rone Aparecido De Grandis; Patrícia Bento da Silva; Anne Lígia Dokkedal; Marlus Chorilli; Tais Maria Bauab; Fernando Rogério Pavan; Eliane Trovatti; Wilton Rogério Lustri; Flávia Ap. Resende

doi:10.1055/a-1323-3622

Planta Medica, Table of Contents

Planta Med 2022; 88(05): 405-415
DOI: 10.1055/a-1323-3622

Formulation and Delivery Systems of Natural Products

Original Papers

Hydroalcoholic Extract of Myrcia bella Loaded into a Microemulsion System: A Study of Antifungal and Mutagenic Potential

Gabriel Davi Marena

¹University of Araraquara (UNIARA), Department of Biological Sciences and Health, Araraquara, São Paulo State, Brazil

,

Luiza Girotto

¹University of Araraquara (UNIARA), Department of Biological Sciences and Health, Araraquara, São Paulo State, Brazil

,

Luiz Leonardo Saldanha

²São Paulo State University (UNESP), Department of Biological Sciences, Faculty of Science, Bauru, São Paulo State, Brazil

,

Matheus Aparecido dos Santos Ramos

³São Paulo State University (UNESP), Department of Biological Sciences, School of Pharmaceutical Sciences, Campus Araraquara, São Paulo State, Brazil

,

Rone Aparecido De Grandis

³São Paulo State University (UNESP), Department of Biological Sciences, School of Pharmaceutical Sciences, Campus Araraquara, São Paulo State, Brazil

,

Patrícia Bento da Silva

⁴São Paulo State University (UNESP), Department of Drugs and Medicines, School of Pharmaceutical Sciences, Campus Araraquara, São Paulo State, Brazil

⁵University of Brasilia, Nanobiotechnology Laboratory, Institute of Biological Sciences, Department of Genetics and Morphology, Brasília, Brazil

,

Anne Lígia Dokkedal

²São Paulo State University (UNESP), Department of Biological Sciences, Faculty of Science, Bauru, São Paulo State, Brazil

,

Marlus Chorilli

⁴São Paulo State University (UNESP), Department of Drugs and Medicines, School of Pharmaceutical Sciences, Campus Araraquara, São Paulo State, Brazil

,

Tais Maria Bauab

³São Paulo State University (UNESP), Department of Biological Sciences, School of Pharmaceutical Sciences, Campus Araraquara, São Paulo State, Brazil

,

Fernando Rogério Pavan

³São Paulo State University (UNESP), Department of Biological Sciences, School of Pharmaceutical Sciences, Campus Araraquara, São Paulo State, Brazil

,

Eliane Trovatti

¹University of Araraquara (UNIARA), Department of Biological Sciences and Health, Araraquara, São Paulo State, Brazil

,

Wilton Rogério Lustri

¹University of Araraquara (UNIARA), Department of Biological Sciences and Health, Araraquara, São Paulo State, Brazil

,

Flávia Ap. Resende

¹University of Araraquara (UNIARA), Department of Biological Sciences and Health, Araraquara, São Paulo State, Brazil

› Author Affiliations

Abstract

Myrcia bella is a medicinal plant used for the treatment of diabetes, hemorrhages, and hypertension in Brazilian folk medicine. Considering that plant extracts are attractive sources of new drugs, the aim of the present study was to verify the influence of incorporating 70% hydroalcoholic of M. bella leaves in nanostructured lipid systems on the mutagenic and antifungal activities of the extract. In this work, we evaluated the antifungal potential of M. bella loaded on the microemulsion against Candida sp for minimum inhibitory concentration, using the microdilution technique. The system was composed of polyoxyethylene 20 cetyl ether and soybean phosphatidylcholine (10%), grape seed oil, cholesterol (10%: proportion 5/1), and purified water (80%). To investigate the mutagenic activity, the Ames test was used with the Salmonella Typhimurium tester strains. M. bella, either incorporated or free, showed an important antifungal effect against all tested strains. Moreover, the incorporation surprisingly inhibited the mutagenicity presented by the extract. The present study attests the antimicrobial properties of M. bella extract, contributing to the search for new natural products with biological activities and suggesting caution in its use for medicinal purposes. In addition, the results emphasize the importance of the use of nanotechnology associated with natural products as a strategy for the control of infections caused mainly by the genus Candida sp.

Key words

Myrcia bella - Myrtaceae - biopharmaceuticals - biotechnology - antifungal - nanotechnology - mutagenicity

Full Text

References

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