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CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(01): e19-e24
DOI: 10.1055/a-1395-6510
Original Papers

Isolation of Adenosine and Cordysinin B from Anredera cordifolia that Stimulates CRE-Mediated Transcription in PC12 Cells

Yasushi Ohizumi
1   Kansei Fukushi Research Institute, Tohoku Fukushi University, Aoba-ku, Sendai, Japan
,
Michi Kawada
1   Kansei Fukushi Research Institute, Tohoku Fukushi University, Aoba-ku, Sendai, Japan
,
Maki Kamada
1   Kansei Fukushi Research Institute, Tohoku Fukushi University, Aoba-ku, Sendai, Japan
,
Akira Nakajima
1   Kansei Fukushi Research Institute, Tohoku Fukushi University, Aoba-ku, Sendai, Japan
2   Department of Applied Biology and Food Sciences, Faculty of Agriculture and Life Science, Hirosaki University, Hirosaki, Aomori, Japan
,
Koji Kajima
3   Sankyo Co., Ltd., Fuji, Shizuoka, Japan
,
Nobuyuki Uozumi
4   Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University, Sendai, Japan
,
Yasumasa Hara
5   Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba, Japan
,
Yuanqiang Guo
6   State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy, and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China
,
Masami Ishibashi
5   Graduate School of Pharmaceutical Sciences, Chiba University, Chuo-ku, Chiba, Japan
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Abstract

Alzheimer’s disease is a typical neurodegenerative disorder, and its prevention or treatment poses great concern in advanced countries. In our survey of numerous natural resources with neurotrophic activities, we found that Anredera cordifolia improved memory impairment and increased cyclic adenosine monophosphate (AMP) response element-mediated transcription, an important step in signal transduction for memory formation. The extracts of this food were dissolved in methanol and then partitioned with three organic solvents and water, separating into n-hexane, ethyl acetate, n-butanol, and water layers. The n-butanol layer with the strongest activity on cyclic AMP-response element-dependent transcription was fractionated using silica gel column chromatography and then the activity was monitored using preparative high-performance liquid chromatography to give adenosine and cordysinin B, respectively. Both compounds showed a concentration-dependent increase in cyclic AMP-response element-mediated transcription activity. These results suggest that both adenosine and cordysinin B may participate in improving the action of A. cordifolia on memory impairment, and these actions, at least in part, result from the activation of adenosine A1, A2A, and A2B receptors.

Key words

Anredera cordifolia - Basellaceae - adenosine - cordysinin B - dementia - CRE-mediated transcription

Supplementary Material



Publication History

Received: 08 October 2020
Received: 12 January 2021

Accepted: 17 February 2021

Article published online:
29 March 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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