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Planta Med 2022; 88(03/04): 282-291
DOI: 10.1055/a-1527-9715
Biological and Pharmacological Activity
Original Papers

Functional and Mechanistic Studies of Two Anti-coccidial Herbs, Bidens pilosa and Artemisia indica

Meng-Ting Yang
1   Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
,
Yi-Xian Lin
1   Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
,
Greta Yang
1   Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
,
Tien-Fen Kuo
1   Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
,
Yu-Chen Liang
1   Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
,
Tsung-Han Lee
2   Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan
,
Cicero Lee-Tian Chang
3   Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, Taichung, Taiwan
,
Wen-Chin Yang
1   Agricultural Biotechnology Research Center, Academia Sinica, Taipei, Taiwan
2   Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan
4   Translational Medicine Research Center, Academia Sinica, Taipei, Taiwan
5   Graduate Institute of Integrated Medicine, China Medical University, Taichung, Taiwan
› Author AffiliationsSupported by: Ministry of Science and Technology MOST 107-2321-B-001-038 -MY3
Supported by: Council of Agriculture 110AS-22.1.2-L1(8)
Supported by: Academia Sinica AS-KPQ-110-ITAR-14
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Abstract

Currently, antibiotics are commonly used to treat coccidiosis, a severe protozoal disease in chickens. However, due to growing concerns about the antibiotic residue in meat and eggs, phytogenic formulations are becoming an attractive approach to manage this disease. In this study, we investigated the anti-coccidial function and mechanism of phytogenic formulations composed of Bidens pilosa, Artemisia indica, and both used in combination. We found that these formulations increased the survival rate and reduced body weight loss, the feed conversion ratio, oocyst excretion, bloody stools, and gut lesions of chickens. Mechanistic studies showed that A. indica, but not B. pilosa, reduced the survival of Eimeria oocysts. Accordingly, they both inhibited oocyst sporulation and sporozoite invasion into Madin-Darby bovine kidney (MDBK) cells. Overall, we demonstrate that these formulations protect chickens against coccidiosis. Moreover, a combination of B. pilosa and A. indica has an additive effect on coccidiosis control and growth performance in chickens compared to either one used alone.

Key words

Coccidiosis - chicken - Asteraceae - Bidens pilosa - Artemisia indica - Eimeria oocysts

Supporting Information

    The supporting information contains HPLC profiles, ultraviolet spectra and mass spectra.

  • Supporting Information


Publication History

Received: 26 February 2021

Accepted after revision: 24 May 2021

Article published online:
29 June 2021

© 2021. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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