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DOI: 10.1055/a-1538-9883
New Cleavable Spacers for Tandem Synthesis of Multiple Oligonucleotides
K.Y. acknowledges a Nagai Memorial Research Scholarship from the Pharmaceutical Society of Japan.
Abstract
In solid-phase oligonucleotide synthesis, a single oligonucleotide is generally acquired from a column loaded with a specific solid support. Herein, we have developed new cleavable spacer (CS) derivatives for tandem synthesis of multiple oligonucleotides on a single column. Four CS analogues were designed, synthesized, and inserted between two oligonucleotide sequences using an automated oligonucleotide synthesizer. The CS derivatives bearing a cyclic cis-1,2-diol exhibited efficient release of the two oligonucleotides under commonly employed basic conditions of aqueous ammonia. Among the CS analogues, it was found that CS with a robust structure can potentially be applied as a spacer molecule in the tandem synthesis of multiple oligonucleotides in a single sequence.
Key words
oligonucleotide synthesis - tandem synthesis - cleavable spacers - phosphoramidites - 1,2-diol derivativesSupporting Information
- Supporting information for this article is available online at https://doi.org/10.1055/a-1538-9883.
- Supporting Information
Publication History
Received: 31 May 2021
Accepted after revision: 28 June 2021
Accepted Manuscript online:
28 June 2021
Article published online:
02 August 2021
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