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Horm Metab Res 2021; 53(10): 699-704
DOI: 10.1055/a-1625-6296
Endocrine Research

Impact of Dietary Sodium Reduction on the Development of Obesity and Type 2 Diabetes in db/db Mice

Anja Hofmann
1   Division of Vascular Endothelium and Microcirculation, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
2   Division of Vascular and Endovascular Surgery, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
,
Coy Brunssen
1   Division of Vascular Endothelium and Microcirculation, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
,
Mirko Peitzsch
3   Institute of Clinical Chemistry and Laboratory Medicine, University Hospital and Faculty of Medicine Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany
,
Jennifer Mittag
1   Division of Vascular Endothelium and Microcirculation, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
,
Annika Frenzel
1   Division of Vascular Endothelium and Microcirculation, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
,
Graeme Eisenhofer
3   Institute of Clinical Chemistry and Laboratory Medicine, University Hospital and Faculty of Medicine Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany
4   Department of Medicine III, University Hospital and Faculty of Medicine Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany
,
Nicholas F. Brown
5   Cardio Metabolic Diseases, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA
,
Steven M. Weldon
5   Cardio Metabolic Diseases, Boehringer Ingelheim Pharmaceuticals Inc., Ridgefield, CT, USA
,
Christian Reeps
2   Division of Vascular and Endovascular Surgery, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
,
Stefan R. Bornstein
4   Department of Medicine III, University Hospital and Faculty of Medicine Carl Gustav Carus Dresden, Technische Universität Dresden, Dresden, Germany
6   Endocrinology and Diabetes, Faculty of Life Sciences & Medicine, Kings College London, London, UK
,
Henning Morawietz
1   Division of Vascular Endothelium and Microcirculation, University Hospital and Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Dresden, Germany
› Author Affiliations Funding Information This work was supported by a research grant from Boehringer Ingelheim and in part by funding of the Excellence Initiative by the German Federal and State Governments (Institutional Strategy, measure "support the best" to H.M.), Doktor Robert Pfleger-Stiftung (to H.M.), and Else Kröner-Fresenius-Stiftung (Grant 2010_A105 to H.M.). Nicholas Brown and Steven M. Weldon are employees of Boehringer Ingelheim and thus contributed to study design, data analysis, and preparation of the manuscript.
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Abstract

The impact of dietary sodium reduction on mouse models of type 2 diabetes is not well understood. Therefore, we analyzed the effect of a low-salt diet on obesity and parameters of type 2 diabetes in db/db mice. Five-week-old male db/db and lean db/m mice were fed a normal salt (0.19% Na+, NS) or a low-salt diet (<0.03% Na+, LS) for 5 weeks. Body and organ weight and parameters of glucose and insulin tolerance were analyzed. Plasma levels of steroids were determined by liquid chromatography tandem mass spectrometry. Body weight, glucose, and insulin tolerance were not affected by LS. The amount of gonadal adipose tissue showed a trend to be increased by LS whereas liver, pancreas, kidney, heart, and adrenal weight remained unaffected. LS reduced urinary sodium-to-creatinine ratio but did not affect plasma Na+ levels in both genotypes. Plasma and urinary potassium-to-creatinine ratio did not differ in all groups of mice. Aldosterone as a major determinant of changes in dietary sodium remained unaffected by LS in db/db mice as well as further investigated steroid hormones. The present study showed reduced sodium-to-creatinine ratio, but no additional effects of dietary sodium reduction on major metabolic parameters and steroid levels in obese and hyper-glycemic db/db mice.

Key words

adipose tissue - aldosterone - diabetes - insulin tolerance - sodium - steroids


Publication History

Received: 24 June 2021

Accepted after revision: 25 August 2021

Article published online:
04 October 2021

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