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CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(03): e153-e160
DOI: 10.1055/a-1648-8111
Original Papers

Effect of Environmental Factors on Plectranthus Neochilus Volatile Composition: A GC-MS-Based Metabolomics Approach

Maria Isabel Galbiatti
1   Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil
,
Guilherme Perez Pinheiro
1   Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil
,
Elisa Ribeiro Miranda Antunes
1   Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, São Paulo, Brazil
,
Vinicius Verri Hernandes
2   ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State University of Campinas, Campinas, São Paulo, Brazil
,
Alexandra Christine Helena Frankland Sawaya
3   Faculty of Pharmaceutical Sciences, State University of Campinas, Campinas, São Paulo, Brazil
› Author Affiliations Funding The authors would like to thank the National Council for Scientific and Technological Development (CNPq Process No. 305298/2017– 8, 132849/2018–6, and 305298/2018–8) and CAPES 001 for funding.
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Abstract

Plectranthus neochilus Schltr. is an aromatic species, commonly used for digestive, antispasmodic, and analgesic purposes. Although many studies have reported the chemical composition of its essential oil, variations in the volatile profile were observed, which may be due to multiple factors linked to growth and field conditions. In order to detect metabolic variations in this species, we employed a GC-MS-based untargeted metabolomics approach analyzing samples of four P. neochilus individuals collected over a year. From all analyses, 24 mass features were detected and 21 were identified according to their respective chromatographic peaks. All features varied among samples, particularly (2E)-hexenal, 3-octanone and δ-3-carene, which showed the highest coefficient of variation percentage in our study. Although the four individuals presented the same peaks in the chromatograms, significant differences in the intensity of specific mass features were detected between individuals throughout the year. Time of sampling did not affect P. neochilus volatile composition; the chemical profile remained constant throughout the day. Seasonal trends were observed for the species. Winter months coincided with a drop in the intensity of most components. Air temperature showed a positive correlation with some feature intensities, while myrcene and α-thujene resulted in a positive and a negative correlation with rainfall, respectively. This study was the first attempt to correlate metabolic variation and environmental factors in P. neochilus. Our approach was successful in identifying the composition and variation of the headspace volatiles of P. neochilus leaves.

Key words

medicinal plants - Plectranthus neochilus - Lamiaceae - plant metabolomics - volatile composition - headspace solid-phase microextraction

Supplementary Material



Publication History

Received: 22 April 2021
Received: 09 July 2021

Accepted: 13 August 2021

Article published online:
20 December 2021

© 2021. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

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