CC BY-NC-ND 4.0 · Planta Medica International Open 2021; 8(03): e153-e160 DOI: 10.1055/a-1648-8111
Original Papers
Effect of Environmental Factors on Plectranthus Neochilus
Volatile Composition: A GC-MS-Based Metabolomics Approach
Maria Isabel Galbiatti
1
Department of Plant Biology, Institute of Biology, State University of
Campinas, Campinas, São Paulo, Brazil
,
Guilherme Perez Pinheiro
1
Department of Plant Biology, Institute of Biology, State University of
Campinas, Campinas, São Paulo, Brazil
,
Elisa Ribeiro Miranda Antunes
1
Department of Plant Biology, Institute of Biology, State University of
Campinas, Campinas, São Paulo, Brazil
,
Vinicius Verri Hernandes
2
ThoMSon Mass Spectrometry Laboratory, Institute of Chemistry, State
University of Campinas, Campinas, São Paulo, Brazil
,
Alexandra Christine Helena Frankland Sawaya
3
Faculty of Pharmaceutical Sciences, State University of Campinas,
Campinas, São Paulo, Brazil
› Author AffiliationsFunding
The authors would like to thank the National Council for Scientific
and Technological Development (CNPq Process No. 305298/2017–
8, 132849/2018–6, and 305298/2018–8) and CAPES 001 for
funding.
Plectranthus neochilus Schltr. is an aromatic species, commonly used for
digestive, antispasmodic, and analgesic purposes. Although many studies have
reported the chemical composition of its essential oil, variations in the
volatile profile were observed, which may be due to multiple factors linked to
growth and field conditions. In order to detect metabolic variations in this
species, we employed a GC-MS-based untargeted metabolomics approach analyzing
samples of four P. neochilus individuals collected over a year. From all
analyses, 24 mass features were detected and 21 were identified according to
their respective chromatographic peaks. All features varied among samples,
particularly (2E)-hexenal, 3-octanone and δ-3-carene,
which showed the highest coefficient of variation percentage in our study.
Although the four individuals presented the same peaks in the chromatograms,
significant differences in the intensity of specific mass features were detected
between individuals throughout the year. Time of sampling did not affect P.
neochilus volatile composition; the chemical profile remained constant
throughout the day. Seasonal trends were observed for the species. Winter months
coincided with a drop in the intensity of most components. Air temperature
showed a positive correlation with some feature intensities, while myrcene and
α-thujene resulted in a positive and a negative correlation
with rainfall, respectively. This study was the first attempt to correlate
metabolic variation and environmental factors in P. neochilus. Our
approach was successful in identifying the composition and variation of the
headspace volatiles of P. neochilus leaves.
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