Feasability of An Eccentric Isokinetic Protocol to Induce Trunk Muscle Damage: A Pilot Study

Abstract

Eccentric exercise is discussed as a treatment option for clinical populations, but specific responses in terms of muscle damage and systemic inflammation after repeated loading of large muscle groups have not been conclusively characterized. Therefore, this study tested the feasibility of an isokinetic protocol for repeated maximum eccentric loading of the trunk muscles. Nine asymptomatic participants (5 f/4 m; 34±6 yrs; 175±13 cm; 76±17 kg) performed three isokinetic 2-minute all-out trunk strength tests (1x concentric (CON), 2x eccentric (ECC1, ECC2), 2 weeks apart; flexion/extension, 60°/s, ROM 55°). Outcomes were peak torque, torque decline, total work, and indicators of muscle damage and inflammation (over 168 h). Statistics were done using the Friedman test (Dunn’s post-test). For ECC1 and ECC2, peak torque and total work were increased and torque decline reduced compared to CON. Repeated ECC bouts yielded unaltered torque and work outcomes. Muscle damage markers were highest after ECC1 (soreness 48 h, creatine kinase 72 h; p<0.05). Their overall responses (area under the curve) were abolished post-ECC2 compared to post-ECC1 (p<0.05). Interleukin-6 was higher post-ECC1 than CON, and attenuated post-ECC2 (p>0.05). Interleukin-10 and tumor necrosis factor-α were not detectable. All markers showed high inter-individual variability. The protocol was feasible to induce muscle damage indicators after exercising a large muscle group, but the pilot results indicated only weak systemic inflammatory responses in asymptomatic adults.

Key words

exercise, - eccentric, - muscle fatigue, - trunk muscles, - isokinetics, - repeated bout effect, - inflammation, - exercise induced muscle damage, - interleukin-6, - internleukin-10, - tumor necrosis factor-α

Publication History

Received: 07 September 2021
Received: 19 October 2021

Accepted: 22 November 2021

Article published online:
19 March 2022

• References

• 1 LaStayo P, Marcus R, Dibble L. et al. Eccentric exercise in rehabilitation: safety, feasibility, and application. J Appl Physiol 2014; 116: 1426-1434
• 2 Douglas J, Pearson S, Ross A. et al. Eccentric exercise: physiological characteristics and acute responses. Sports Med 2017; 47: 663-675
• 3 Franchi MV, Reeves ND, Narici MV. Skeletal muscle remodeling in response to eccentric vs. concentric loading: morphological, molecular, and metabolic adaptations. Front Physiol 2017; 8: 447
• 4 Mayer F, Axmann D, Horstmann T. et al. Metabolic and cardiocirculatory reactions after concentric and eccentric exercise of the shoulder. Int J Sports Med 1999; 20: 527-531
• 5 Vallejo AF, Schroeder ET, Zheng L. et al. Cardiopulmonary responses to eccentric and concentric resistance exercise in older adults. Age Ageing 2006; 35: 291-297
• 6 Drexel H, Saely CH, Langer P. et al. Metabolic and anti-inflammatory benefits of eccentric endurance exercise – a pilot study. Eur J Clin Invest 2008; 38: 218-226
• 7 Zeppetzauer M, Drexel H, Vonbank A. et al. Eccentric endurance exercise economically improves metabolic and inflammatory risk factors. Eur J Prev Cardiol 2013; 20: 577-584
• 8 Philippe M, Junker G, Gatterer H. et al. Acute effects of concentric and eccentric exercise matched for energy expenditure on glucose metabolism in healthy females: a randomized crossover trial. Springerplus 2016; 5: 1455
• 9 Nascimento DdC, Navalta JW, Durigan JLQ. et al. Acute eccentric resistance exercise decreases matrix metalloproteinase activity in obese elderly women. Clin Physiol Funct Imaging 2016; 36: 139-145
• 10 Hyldahl RD, Hubal MJ. Lengthening our perspective: morphological, cellular, and molecular responses to eccentric exercise. Muscle Nerve 2014; 49: 155-170
• 11 Hyldahl RD, Chen TC, Nosaka K. Mechanisms and mediators of the skeletal muscle repeated bout effect. Exerc Sport Sci Rev 2017; 45: 24-33
• 12 Peake J, Nosaka K, Suzuki K. Characterization of inflammatory responses to eccentric exercise in humans. Exerc Immunol Rev 2005; 11: 64-85
• 13 Suzuki K. Characterization of exercise-induced cytokine release, the impacts on the body, the mechanisms and modulations. Int J Sports Exerc Med 2019; 5
• 14 Croisier JL, Camus G, Venneman I. et al. Effects of training on exercise-induced muscle damage and interleukin 6 production. Muscle Nerve 1999; 22: 208-212
• 15 Willoughby DS, McFarlin B, Bois C. Interleukin-6 expression after repeated bouts of eccentric exercise. Int J Sports Med 2003; 24: 15-21
• 16 Peake JM, Suzuki K, Wilson G. et al. Exercise-induced muscle damage, plasma cytokines, and markers of neutrophil activation. Med Sci Sports Exerc 2005; 37: 737-745
• 17 Hirose L, Nosaka K, Newton M. et al. Changes in inflammatory mediators following eccentric exercise of the elbow flexors. Exerc Immunol Rev 2004; 10: 75-90
• 18 Conceição MS, Libardi CA, Nogueira FRD. et al. Effects of eccentric exercise on systemic concentrations of pro- and anti-inflammatory cytokines and prostaglandin (E2): comparison between young and postmenopausal women. Eur J Appl Physiol 2012; 112: 3205-3213
• 19 Cornish SM, Johnson ST. Systemic cytokine response to three bouts of eccentric exercise. Results Immunol 2014; 4: 23-29
• 20 Kanda K, Sugama K, Hayashida H. et al. Eccentric exercise-induced delayed-onset muscle soreness and changes in markers of muscle damage and inflammation. Exerc Immunol Rev 2013; 19: 72-85
• 21 Chen TC, Yang T-J, Huang M-J. et al. Damage and the repeated bout effect of arm, leg, and trunk muscles induced by eccentric resistance exercises. Scand J Med Sci Sports 2019; 29: 725-735
• 22 Harriss DJ, MacSween A, Atkinson G. Ethical standards in sport and exercise science research: 2020 update. Int J Sports Med 2019; 40: 813-817
• 23 Mueller J, Mueller S, Stoll J. et al. Trunk extensor and flexor strength capacity in healthy young elite athletes aged 11–15 years. J Strength Cond Res 2014; 28: 1328-1334
• 24 Mueller S, Engel T, Mueller J. et al. Sensorimotor exercises and enhanced trunk function: a randomized controlled trial. Int J Sports Med 2018; 39: 555-563
• 25 Pasquet B, Carpentier A, Duchateau J. et al. Muscle fatigue during concentric and eccentric contractions. Muscle Nerve 2000; 23: 1727-1735
• 26 Goodall S, Thomas K, Barwood M. et al. Neuromuscular changes and the rapid adaptation following a bout of damaging eccentric exercise. Acta Physiol (Oxf) 2017; 220: 486-500
• 27 Reeves ND, Maganaris CN, Longo S. et al. Differential adaptations to eccentric versus conventional resistance training in older humans. Exp Physiol 2009; 94: 825-833
• 28 Henriksson J, Knuttgen HG, Bonde-Petersen F. Perceived exertion during exercise with concentric and eccentric muscle contractions. Ergonomics 1972; 15: 537-544
• 29 Scalco RS, Snoeck M, Quinlivan R. et al. Exertional rhabdomyolysis: physiological response or manifestation of an underlying myopathy?. BMJ Open Sport Exerc Med 2016; 2: e000151
• 30 Tajra V, Tibana RA, Vieira DCL. et al. Identification of high responders for interleukin-6 and creatine kinase following acute eccentric resistance exercise in elderly obese women. J Sci Med Sport 2014; 17: 662-666
• 31 Chen TC. Variability in muscle damage after eccentric exercise and the repeated bout effect. Res Q Exerc Sport 2006; 77: 362-371
• 32 Brown SJ, Child RB, Day SH. et al. Indices of skeletal muscle damage and connective tissue breakdown following eccentric muscle contractions. Eur J Appl Physiol Occup Physiol 1997; 75: 369-374
• 33 Chen TC, Nosaka K. Effects of number of eccentric muscle actions on first and second bouts of eccentric exercise of the elbow flexors. J Sci Med Sport 2006; 9: 57-66
• 34 Sproston NR, Ashworth JJ. Role of C-reactive protein at sites of inflammation and infection. Front Immunol 2018; 9
• 35 Afroundeh R, Siahkouhian M, Khalili A. The effect of post-exercise carbohydrate ingestion on inflammatory responses to short time, high-force eccentric exercise. J Sports Med Phys Fitness 2010; 50: 182-188
• 36 Phillips T, Childs AC, Dreon DM. et al. A dietary supplement attenuates IL-6 and CRP after eccentric exercise in untrained males. Med Sci Sports Exerc 2003; 35: 2032-2037
• 37 Deyhle MR, Gier AM, Evans KC. et al. Skeletal muscle inflammation following repeated bouts of lengthening contractions in humans. Front Physiol 2016; 6: 424
• 38 Smith LL, McKune AJ, Semple SJ. et al. Changes in serum cytokines after repeated bouts of downhill running. Appl Physiol Nutr Metab 2007; 32: 233-240
• 39 Toft AD, Jensen LB, Bruunsgaard H. et al. Cytokine response to eccentric exercise in young and elderly humans. Am J Physiol Cell Physiol 2002; 283: C289-C295
• 40 Peake JM, Suzuki K, Hordern M. et al. Plasma cytokine changes in relation to exercise intensity and muscle damage. Eur J Appl Physiol 2005; 95: 514-521
• 41 Mavropalias G, Koeda T, Barley OR. et al. Comparison between high- and low-intensity eccentric cycling of equal mechanical work for muscle damage and the repeated bout effect. Eur J Appl Physiol 2020; 120: 1015-1025
• 42 McGavock JM, Mandic S, Vonder Muhll I. et al. Low cardiorespiratory fitness is associated with elevated C-reactive protein levels in women with type 2 diabetes. Diabetes Care 2004; 27: 320-325
• 43 Petersen AMW, Pedersen BK. The anti-inflammatory effect of exercise. J Appl Physiol 2005; 98: 1154-1162
• 44 Julian V, Thivel D, Costes F. et al. Eccentric training improves body composition by inducing mechanical and metabolic adaptations: a promising approach for overweight and obese individuals. Front Physiol 2018; 9: 1013
• 45 Chen TC, Hsieh C-C, Tseng K-W. et al. Effects of descending stair walking on health and fitness of elderly obese women. Med Sci Sports Exerc 2017; 49: 1614-1622
• 46 Chen TC-C, Tseng W-C, Huang G-L. et al. Superior effects of eccentric to concentric knee extensor resistance training on physical fitness, insulin sensitivity and lipid profiles of elderly men. Front Physiol 2017; 8: 209