Abstract
Acute kidney injury (AKI) is a major medical challenge caused from renal
ischemia-reperfusion (IR) injury connected with different cellular events in
other distant organs. Renal IR-related oxidative stress and inflammation
followed by cell apoptosis play a crucial role in IR-induced distant organ
pathological damages. Prazosin has shown protective effects against IR-injuries.
Thus, the current study intended to investigate the possible protective role of
prazosin against the consequents of renal IR in the heart and brain tissues. To
reach this goal, rats were randomly divided into 3 groups (n=7): Sham,
IR and prazosin pretreatment-IR animals (1 mg/kg
intraperitoneally injection of prazosin 45 min before IR induction).
After 6 h reperfusion, lipid peroxidation and antioxidant markers levels
were evaluated in the both, brain and heart tissue. Moreover, apoptotic pathway
in the heart and brain tissues were assessed by western blotting. Accordingly,
prazosin pretreatment in IR model rats could significantly increase the
antioxidant capacity and attenuate apoptotic pathways by increasing the bcl-2
levels and decreasing the expression of Bax and caspase 3 enzymes
(P<0.05). Thus, prazosin suppressed cellular damages of heart and brain
tissues post kidney IR by anti-oxidative and anti-apoptotic effects, which
suggests the plausible use of prazosin in improving the clinical outcomes during
AKI after further investigations.
Key words
Renal ischemia reperfusion - multi-organ dysfunction - oxidative stress - acute kidney injury - Prazosin
