MiR-99a-5p Inhibits the Proliferation and Migration of Human Retinal
                    Microvascular Endothelial Cells by Targeting NOX4

Haizhen Yu; Xu Zhang; Xuyang Wang; Wangling Chen; Wei Lao; Yunxin Chen

doi:10.1055/a-1982-3926

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 2023; 55(02): 142-148
DOI: 10.1055/a-1982-3926

Original Article: Endocrine Research

MiR-99a-5p Inhibits the Proliferation and Migration of Human Retinal Microvascular Endothelial Cells by Targeting NOX4

Haizhen Yu⁺

¹Department of Clinical Laboratory, Zhucheng People’s Hospital, Weifang, China

,

Xu Zhang⁺

²Department of Ophthalmology, Shanghai Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China

,

Xuyang Wang

³Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Opthalmic Center, Sun Yat-sen University, Haikou, Hainan, China

,

Wangling Chen

³Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Opthalmic Center, Sun Yat-sen University, Haikou, Hainan, China

,

Wei Lao

³Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Opthalmic Center, Sun Yat-sen University, Haikou, Hainan, China

,

Yunxin Chen

³Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Opthalmic Center, Sun Yat-sen University, Haikou, Hainan, China

› Institutsangaben

Abstract

Diabetic retinopathy is one of the common microvascular complications of diabetes, and it is the main cause of vision loss among working-age people. This study interpreted the roles of miR-99a-5p in DR patients and human retinal microvascular endothelial cell (hRMECs) injury induced by high glucose. The expression of miR-99a-5p was detected in patients with NDR, NPDR, and PDR. The indictive impacts of miR-99a-5p were tested by the ROC curve, and the link between miR-99a-5p and clinical information was verified by the Pearson test. HG was used to instruct cell models. The CCK-8 and transwell methods were performed to detect the proliferative and migrated cells. The targeted relationship was explained by luciferase activity. The content of miR-99a-5p was gradually lessened in NPDR and PDR patients. MiR-99a-5p might differentiate DR patients from NDR patients and PDR patients from NPDR patients. The interconnection between miR-99a-5p and clinical factors was endorsed in all DR patients. Overexpression of miR-99a-5p assuaged the abnormality of cell migration and proliferation of hRMECs triggered by HG. NOX4 was a downstream signaling component of miR-99a-5p. In conclusion, MiR-99a-5p protected hRMECs against HG damage, and the miR-99a-5p might be a novel target for diagnosis of DR.

Key words

miR-99a-5p - diagnosis - diabetic retinopathy - NOX4

Volltext

Referenzen

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