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Drug Res (Stuttg) 2023; 73(05): 255-262
DOI: 10.1055/a-1999-7600
Original Article

The Effects of Quercetin on Apoptosis and Antioxidant Activity in a Renal Ischemia/Reperfusion Injury Animal Model

Amin Bagheri
1   Department of Urology, Sina Hospital, Tabriz University of Medical Sciences, Tabriz, Iran
,
Ghazal Radman
2   Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
3   Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal Scientific Education and Research Network (USERN), Tehran, Iran
,
Negar Aria
4   Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran
,
Fatemeh Rezaei
5   Faculty of Pharmacy in Hradec Kralove, Charles University, Prague, Czech Republic
,
Mohammad Khajenouri
6   Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
,
Shamim Ghiabi
7   Department of Medical Chemistry, Faculty of Pharmacy, Tehran Medical Sciences Islamic Azad University, Tehran, Iran
,
Yasin Bagheri
8   Kidney Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
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Abstract

Renal ischemia-reperfusion injury (IRI) is considered as one of the most prevalent causes of acute kidney injury (AKI), which can happen in various clinical situations including hypovolemic shock, injury, thrombo-embolism, and after a kidney transplant. This paper aims to evaluate the reno-protective effects of Quercetin in induced ischemia/reperfusion injury by regulating apoptosis-related proteins, inflammatory cytokines, MMP-2, MMP-9, and nuclear factor kappa-light-chain-enhancer inactivated B cells (NF-kB) in rats. The male Wistar rats (n=32) were randomly divided into Sham, untreated IR, and Quercetin-treated IR (gavage and intraperitoneal). Quercetin was given orally and intraperitoneally one hour before inducing ischemia-reperfusion injury . After reperfusion, blood samples and kidneys were collected to assess renal function and inflammatory cytokines, apoptotic signaling proteins, and antioxidants. Urea, creatinine, and MDA levels improved in Quercetin-treated groups with different administration methods. In addition, the activities of other antioxidant in Quercetin-treated rats were higher than those in the IR group. Further, Quercetin inhibited NF-kB signaling, apoptosis-associated factors and produced matrix metalloproteinase protein in the kidneys of rats. Based on the findings, the antioxidant, anti-inflammatory, and anti-apoptotic effects of the Quercetin diminished renal ischemia-reperfusion injury in the rats significantly. It is suggested that a single dosage of Quercetin have a reno-protective impact in the case of renal I/R injury.

Keywords

Quercetin - acute renal injury - ischemia-reperfusion - oxidative stress - apoptosis


Publication History

Received: 06 September 2022

Accepted: 12 December 2022

Article published online:
27 March 2023

© 2023. Thieme. All rights reserved.

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