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Am J Perinatol 2024; 41(10): 1388-1395
DOI: 10.1055/a-2033-5536
Original Article

Correlation of Polymorphonuclear Cell Burden and Microbial Growth to the Inflammatory Cytokines in Tracheal Aspirates from Ventilated Preterm Infants

Sophia Baig
1   Section of Neonatal-Perinatal Medicine, Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Philadelphia, Pennsylvania
,
Pragnya Das
1   Section of Neonatal-Perinatal Medicine, Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Philadelphia, Pennsylvania
,
Niharika Podaralla
1   Section of Neonatal-Perinatal Medicine, Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Philadelphia, Pennsylvania
,
Alan Evangelista
2   Department of Pathology & Laboratory Medicine, Drexel University College of Medicine, St. Christopher's Hospital for Children, Philadelphia, Pennsylvania
,
Ishminder Kaur
3   Section of Infectious Diseases, Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Philadelphia, Pennsylvania
,
Vineet Bhandari
1   Section of Neonatal-Perinatal Medicine, Department of Pediatrics, Drexel University College of Medicine, St. Christopher's Hospital for Children, Philadelphia, Pennsylvania
› Author Affiliations Funding This work was supported by a grant from St. Christopher's Foundation to S.B.
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Abstract

Objective The significance of the presence of microorganisms and polymorphonuclear cells in the tracheal aspirates (TAs) of ventilated preterm infants is not well known. Our aim was to correlate information about the presence of polymorphonuclear cells with microbial growth and the cytokine milieu in the TAs of infants who have been intubated for >7 days.

Study Design TAs were collected from infants who had been intubated for 7 days or longer. Respiratory cultures were performed, and infants were stratified based on the presence and abundance of polymorphonuclear cells and microbial growth. Cytokines were measured in the TAs of each of the respective groups.

Results In the 19 infants whose TAs were collected, the presence of at least moderate WBC with presence of microbial growth was positively associated with the presence of interleukin (IL)-10, IL-1β, IL-8, and tumor necrosis factor (TNF)-α. The presence of at least moderate WBC, with or without microbial growth, was correlated positively with the presence of IL-8 and TNF-α.

Conclusion There are higher levels of proinflammatory cytokines (especially, IL-10, IL-1β, and TNF-α) in TAs with higher cell counts and presence of microbial growth. The findings suggest that the presence of microbial growth correlated with inflammatory burden and warrant a larger study to see if treatment of microbial growth can ameliorate the inflammatory burden.

Key Points

  • Concomitant evaluation of inflammatory cells, microbial growth, and cytokines in tracheal aspirates.

  • Moderate TA WBC with presence of microbial growth associated with IL-10, IL-1β, IL-8, and TNF-α.

  • Moderate TA WBC, with/without microbial growth, correlated with the presence of IL-8 and TNF-α.

  • Higher levels of IL-10, IL-1β, and TNF-α correlated with higher TA cell counts and microbial growth.

Keywords

cytokines - cell counts - tracheal aspirate cultures - preterm newborn

Supplementary Material



Publication History

Received: 26 October 2022

Accepted: 11 February 2023

Accepted Manuscript online:
11 February 2023

Article published online:
07 March 2023

© 2023. Thieme. All rights reserved.

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