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Int J Sports Med 2023; 44(08): 584-591
DOI: 10.1055/a-2044-8691
Training & Testing

Effects of Exercise Intensity on Cardiometabolic Parameters of Ovariectomized Obese Mice

Adriano Dos-Santos
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Bruno do Nascimento Carvalho 
2   Heart Institute, department of hypertension, University of Sao Paulo, Faculty of Medicine, Sao Paulo, Brazil
,
Nicolas Da Costa-Santos
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Fernanda Queiroz de Mello-Silva 
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Abel de Assis Pereira
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Ney Roberto de Jesus
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Kátia De Angelis 
3   Department of Medicine, Federal University of Sao Paulo, Brazil
,
Maria Claudia Irigoyen
2   Heart Institute, department of hypertension, University of Sao Paulo, Faculty of Medicine, Sao Paulo, Brazil
,
Nathalia Bernardes
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Erico Chagas Caperuto
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Katia Bilhar Scapini
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
,
Iris Callado Sanches
1   Human Movement Laboratory, Sao Judas Tadeu University, Sao Paulo, Brazil
› Author Affiliations Funding This study was financed by the Coordination for the Improvement of Higher Education Personnel (CAPES), Brazilian National Council for Scientific and Technological Development (CNPq) and Anima Institute (AI). B.N.C., A.S. and N.C.S. were recipients of CAPES-PROSUP scholarship. I.C.S. was recipient of CNPq/Universal (435123/2018–1).
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Abstract

The aim of this study was to compare the effects of continuous-moderate vs. high-intensity interval aerobic training on cardiovascular and metabolic parameters in ovariectomized high-fat-fed mice. C57BL/6 female ovariectomized were divided into four groups (n=8): low-fat-fed sedentary (SLF); high-fat-fed sedentary (SHF); high-fat-fed moderate-intensity continuous trained (MICT-HF); and high-fat-fed high-intensity interval aerobic trained (HIIT-HF). The high-fat diet lasted 10 weeks. Ovariectomy was performed in the fourth week. The exercise training was carried out in the last four weeks of protocol. Fasting glycemia, oral glucose tolerance, arterial pressure, baroreflex sensitivity, and cardiovascular autonomic modulation were evaluated. Moderate-intensity continuous training prevented the increase in arterial pressure and promoted a reduction in HR at rest, associated with an improvement in the sympathovagal balance in MICT-HF vs. SHF. The high-intensity interval training reduced blood glucose and glucose intolerance in HIIT-HF vs. SHF and MICT-HF. In addition, it improved sympathovagal balance in HIIT-HF vs. SHF. Moderate-intensity continuous training was more effective in promoting cardiovascular benefits, while high-intensity interval training was more effective in promoting metabolic benefits.

Key words

menopause - obesity - exercise training - heart rate variability - high-fat diet - high-intensity interval training


Publication History

Received: 13 September 2022

Accepted: 09 February 2023

Article published online:
05 May 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag
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  • References

  • 1 WHO. Obesity and Overweight. World Health Organ 2020; Internet: https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight; Accessed: May 30, 2020
    PubMed
  • 2 Abildgaard J, Ploug T, Al-Saoudi E. et al. Changes in abdominal subcutaneous adipose tissue phenotype following menopause is associated with increased visceral fat mass. Sci Rep 2021; 11: 14750
    CrossrefPubMedGoogle Scholar
  • 3 Abildgaard J, Danielsen ER, Dorph E. et al. Ectopic lipid deposition is associated with insulin resistance in postmenopausal women. J Clin Endocrinol Metab 2018; 103: 3394-3404
    CrossrefPubMedGoogle Scholar
  • 4 Peppa M, Koliaki C, Dimitriadis G. Body composition as an important determinant of metabolic syndrome in postmenopausal women. Endocrinol Metabol Syndrome 2012; S1: 009
    PubMedGoogle Scholar
  • 5 Force T. Heart rate variability – Standards of measurement, physiological interpretation, and clinical use. Eur Heart J 1996; 17: 354-381
    CrossrefPubMedGoogle Scholar
  • 6 De Angelis K, Santos MDSB, Irigoyen MC.. Sistema nervoso autônomo e doença cardiovascular. Revista da Sociedade de Cardiologia do Rio Grande do Sul 2004; 13: 1-7
    PubMedGoogle Scholar
  • 7 Goldmeier S, De Angelis K, Casali KR. et al. Cardiovascular autonomic dysfunction in primary ovarian insufficiency: Clinical and experimental evidence. Am J Transl Res 2014; 6: 91-101
    PubMedGoogle Scholar
  • 8 Mercuro G, Podda A, Pitzalis L. et al. Evidence of a role of endogenous estrogen in the modulation of autonomic nervous system. Am J Cardiol 2000; 85: 787-789
    CrossrefPubMedGoogle Scholar
  • 9 Roh J, Rhee J, Chaudhari V. et al. The role of exercise in cardiac aging: from physiology to molecular mechanisms. Circ Res 2016; 118: 279-295
    CrossrefPubMedGoogle Scholar
  • 10 Carrick-Ranson G, Hastings JL, Bhella PS. et al. The effect of lifelong exercise dose on cardiovascular function during exercise. J Appl Physiol (1985) 2014; 116: 736-745
    CrossrefPubMedGoogle Scholar
  • 11 Whelton PK, Carey RM, Aronow WS. et al. ACC/AHA/AAPA/ABC/ACPM/AGS/APhA/ASH/ASPC/NMA/PCNA Guideline for the prevention, detection, evaluation, and management of high blood pressure in adults: A report of the American College of Cardiology/American Heart Association Task Force on clinical practice guidelines. Hypertension 2018; 71: e13-e115
    PubMedGoogle Scholar
  • 12 Heeren M, De Sousa L, Mostarda C. et al. Exercise improves cardiovascular control in a model of dislipidemia and menopause. Maturitas 2009; 62: 200-204
    CrossrefPubMedGoogle Scholar
  • 13 De Angelis K, Wichi RB, Jesus WRA. et al. Exercise training changes autonomic cardiovascular balance in mice. J Appl Physiol (1985) 2004; 96: 2174-2178
    CrossrefPubMedGoogle Scholar
  • 14 Sanches IC, de Oliveira Brito J, Candido GO. et al. Cardiometabolic benefits of exercise training in an experimental model of metabolic syndrome and menopause. Menopause 2012; 19: 562-568
    CrossrefPubMedGoogle Scholar
  • 15 Flues K, Paulini J, Brito S. et al. Exercise training associated with estrogen therapy induced cardiovascular benefits after ovarian hormones deprivation. Maturitas 2010; 65: 267-271
    CrossrefPubMedGoogle Scholar
  • 16 Oliveira BRR, Santos TM, Kilpatrick M. et al. Affective and enjoyment responses in high intensity interval training and continuous training: A systematic review and meta-analysis. PLoS One 2018; 13: e0197124
    CrossrefPubMedGoogle Scholar
  • 17 Stork MJ, Banfield LE, Gibala MJ. et al. A scoping review of the psychological responses to interval exercise: is interval exercise a viable alternative to traditional exercise?. Health Psychol Rev 2017; 11: 324-344
    CrossrefPubMedGoogle Scholar
  • 18 Trapp EG, Chisholm DJ, Freund J. et al. The effects of high-intensity intermittent exercise training on fat loss and fasting insulin levels of young women. Int J Obes (Lond) 2008; 32: 684-691
    CrossrefPubMedGoogle Scholar
  • 19 Craighead DH, Heinbockel TC, Hamilton MN. et al. Time-efficient physical training for enhancing cardiovascular function in midlife and older adults: Promise and current research gaps. J Appl Physiol (1985) 2019; 127: 1427-1440
    CrossrefPubMedGoogle Scholar
  • 20 Weston KS, Wisløff U, Coombes JS. High-intensity interval training in patients with lifestyle-induced cardiometabolic disease: A systematic review and meta-analysis. Br J Sports Med 2014; 48: 1227-1234
    CrossrefPubMedGoogle Scholar
  • 21 Stoyell-Conti F, Santos F, Machi J. et al. Measurement of mouse heart rate variability using echocardiographic system. J Cardiovasc Echogr 2018; 28: 90-94
    CrossrefPubMedGoogle Scholar
  • 22 Harriss DJ, Jones C, MacSween A. Ethical standards in sport and exercise science research: 2022 update. Int J Sports Med 2022; 43: 1065-1070
    Article in Thieme ConnectPubMedGoogle Scholar
  • 23 Reeves PG, Nielsen FH, Fahey GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993; 123: 1939-1951
    CrossrefPubMedGoogle Scholar
  • 24 Marchon C, de Marco Ornelas E, da Silva Viegas KA. et al. Effects of moderate exercise on the biochemical, physiological, morphological and functional parameters of the aorta in the presence of estrogen deprivation and dyslipidemia: an experimental model. Cell Physiol Biochem 2015; 35: 397-405
    CrossrefPubMedGoogle Scholar
  • 25 Ludgero-Correia A, Aguila MB, Mandarim-de-Lacerda CA. et al. Effects of high-fat diet on plasma lipids, adiposity, and inflammatory markers in ovariectomized C57BL/6 mice. Nutrition 2012; 28: 316-323
    CrossrefPubMedGoogle Scholar
  • 26 Flores LJ, Figueroa D, Sanches IC. et al. Effects of exercise training on autonomic dysfunction management in an experimental model of menopause and myocardial infarction. Menopause 2010; 17: 712-717
    CrossrefPubMedGoogle Scholar
  • 27 Wang N, Liu Y, Ma Y. et al. High-intensity interval versus moderate-intensity continuous training: Superior metabolic benefits in diet-induced obesity mice. Life Sci 2017; 191: 122-131
    CrossrefPubMedGoogle Scholar
  • 28 Abad CCC, do Nascimento AM, dos Santos LE. et al. Interval and continuous aerobic exercise training similarly increase cardiac function and autonomic modulation in infarcted mice. J Exerc Rehabil 2017; 13: 257-265
    CrossrefPubMedGoogle Scholar
  • 29 Campos C, Bertagnolli M, Evangelho J. et al. Baroreflex sensitivity and oxidative stress in the LDL receptor knockout mice. Exp Toxicol Pathol 2008; 60: 329-335
    CrossrefPubMedGoogle Scholar
  • 30 Sanches IC, Sartori M, Jorge L. et al. Tonic and reflex cardiovascular autonomic control in trained-female rats. Braz J Med Biol Res 2009; 42: 942-948
    CrossrefPubMedGoogle Scholar
  • 31 Souza SBC, Flues K, Paulini J. et al. Role of exercise training in cardiovascular autonomic dysfunction and mortality in diabetic ovariectomized rats. Hypertension. 2007. 50. 786-791
    Google Scholar
  • 32 Thireau J, Zhang BL, Poisson D. et al. Heart rate variability in mice: A theoretical and practical guide. Exp Physiol 2008; 93: 83-94
    CrossrefPubMedGoogle Scholar
  • 33 Gehrmann J, Hammer PE, Maguire CT. et al. Phenotypic screening for heart rate variability in the mouse. Am J Physiol Heart Circ Physiol 2000; 279: H733-H740
    CrossrefPubMedGoogle Scholar
  • 34 White PAS, Cercato LM, Araújo JMD. et al. Modelo de obesidade induzida por dieta hiperlipídica e associada à resistência à ação da insulina e intolerância à glicose. Arq Bras Endocrinol Metabol 2013; 57: 339-345
    CrossrefPubMedGoogle Scholar
  • 35 Appiakannan HS, Rasimowicz ML, Harrison CB. et al. Differential effects of high-fat diet on glucose tolerance, food intake, and glucocorticoid regulation in male C57BL/6J and BALB/cJ mice. Physiol Behav 2020; 215: 112773
    CrossrefPubMedGoogle Scholar
  • 36 Prior RL, Wu X, Gu L. et al. Whole berries versus berry anthocyanins: Interactions with dietary fat levels in the C57BL/6J mouse model of obesity. J Agric Food Chem 2008; 56: 647-653
    CrossrefPubMedGoogle Scholar
  • 37 Townsend KL, Lorenzi MM, Widmaier EP. High-fat diet-induced changes in body mass and hypothalamic gene expression in wild-type and leptin-deficient mice. Endocrine 2008; 33: 176-188
    CrossrefPubMedGoogle Scholar
  • 38 Buettner R, Parhofer KG, Woenckhaus M. et al. Defining high-fat-diet rat models: Metabolic and molecular effects of different fat types. J Mol Endocrinol 2006; 36: 485-501
    CrossrefPubMedGoogle Scholar
  • 39 Hariri N, Gougeon R, Thibault L. A highly saturated fat-rich diet is more obesogenic than diets with lower saturated fat content. Nutr Res 2010; 30: 632-643
    CrossrefPubMedGoogle Scholar
  • 40 Marcinko K, Sikkema SR, Samaan MC. et al. High intensity interval training improves liver and adipose tissue insulin sensitivity. Mol Metab 2015; 4: 903-915
    CrossrefPubMedGoogle Scholar
  • 41 Mandrup CM, Egelund J, Nyberg M. et al. Effects of menopause and high-intensity training on insulin sensitivity and muscle metabolism. Menopause 2018; 25: 165-175
    CrossrefPubMedGoogle Scholar
  • 42 Consitt LA, Dudley C, Saxena G. Impact of endurance and resistance training on skeletal muscle glucose metabolism in older adults. Nutrients 2019; 11: 2636
    CrossrefPubMedGoogle Scholar
  • 43 Khalafi M, Ravasi AA, Malandish A. et al. The impact of high-intensity interval training on postprandial glucose and insulin: A systematic review and meta-analysis. Diabetes Res Clin Pract 2022; 186: 109815
    CrossrefPubMedGoogle Scholar
  • 44 Rodrigues B, Figueroa DM, Mostarda CT. et al. Maximal exercise test is a useful method for physical capacity and oxygen consumption determination in streptozotocin-diabetic rats. Cardiovasc Diabetol 2007; 6: 38
    CrossrefPubMedGoogle Scholar
  • 45 Bernstein D. Exercise assessment of transgenic models of human cardiovascular disease. Physiol Genomics 2003; 13: 217-226
    CrossrefPubMedGoogle Scholar
  • 46 Perrino C, Gargiulo G, Pironti G. et al. Cardiovascular effects of treadmill exercise in physiological and pathological preclinical settings. Am J Physiol Heart Circ Physiol 2011; 300: H1983-H1989
    CrossrefPubMedGoogle Scholar
  • 47 Wichi RB, De Angelis K, Jones L. et al. A brief review of chronic exercise intervention to prevent autonomic nervous system changes during the aging process. Clinics (Sao Paulo) 2009; 64: 253-258
    CrossrefPubMedGoogle Scholar
  • 48 Pimenta M, Bringhenti I, Souza-Mello V. et al. High-intensity interval training beneficial effects on body mass, blood pressure, and oxidative stress in diet-induced obesity in ovariectomized mice. Life Sci 2015; 139: 75-82
    CrossrefPubMedGoogle Scholar
  • 49 Sá G, de O, Neves V. et al. High-intensity interval training has beneficial effects on cardiac remodeling through local renin-angiotensin system modulation in mice fed high-fat or high-fructose diets. Life Sci 2017; 189: 8-17
    CrossrefPubMedGoogle Scholar
  • 50 Masson GS, Costa TSR, Yshii L. et al. Time-dependent effects of training on cardiovascular control in spontaneously hypertensive rats: Role for brain oxidative stress and inflammation and baroreflex sensitivity. PLoS One 2014; 9: e94927
    CrossrefPubMedGoogle Scholar
  • 51 Moraes-Silva I, De La Fuente R, Mostarda C. et al. Baroreflex deficit blunts exercise training-induced cardiovascular and autonomic adaptations in hypertensive rats. Clin Exp Pharmacol Physiol 2010; 37: e114-e120
    CrossrefPubMedGoogle Scholar
  • 52 Li G, Liu JY, Zhang HX. et al. Exercise training attenuates sympathetic activation and oxidative stress in diet-induced obesity. Physiol Res 2015; 64: 355-367
    CrossrefPubMedGoogle Scholar
  • 53 Irigoyen MC, De Angelis K, dos Santos F. et al. Hypertension, blood pressure variability, and target organ lesion. Curr Hypertens Rep 2016; 18: 18-31
    CrossrefPubMedGoogle Scholar
  • 54 Caminiti G, Mancuso A, Raposo AF. et al. Different exercise modalities exert opposite acute effects on short-term blood pressure variability in male patients with hypertension. Eur J Prev Cardiol 2019; 26: 1028-1031
    CrossrefPubMedGoogle Scholar
  • 55 Carrard J, Rigort AC, Appenzeller-Herzog C. et al. Diagnosing overtraining syndrome: A scoping review. Sports Health 2022; 14: 665-673
    CrossrefPubMedGoogle Scholar
  • 56 Kajaia T, Maskhulia L, Chelidze K. et al. The effects of Non-Functional overreaching and overtraining on autonomic nervous system function in highly trained athletes. Georgian Med News 2017; 264: 97-103
    PubMedGoogle Scholar
  • 57 Castrillón CIM, Miranda RAT, Cabral-Santos C. et al. High-intensity intermittent exercise and autonomic modulation: Effects of different volume sessions. Int J Sports Med 2017; 38: 468-472
    Article in Thieme ConnectPubMedGoogle Scholar