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Exp Clin Endocrinol Diabetes 2023; 131(05): 282-289
DOI: 10.1055/a-2044-8873
Article

Increased Galactosidase Beta 1 Expression as a Senescent Key Factor in β-Cells Function Modulation at the Early Steps of Type 2 Diabetes

Ana T. Maduro
1   Biochemistry Unit, Department of Biomedicine, Faculty of Medicine, University of Porto, Portugal
2   i3S, Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal
,
Anabela Pinto
3   Experimental Biology Unit, Department of Biomedicine, Faculty of Medicine, University of Porto, Portugal
,
Joana Ferreira-Gomesb
3   Experimental Biology Unit, Department of Biomedicine, Faculty of Medicine, University of Porto, Portugal
,
Raquel Costa
1   Biochemistry Unit, Department of Biomedicine, Faculty of Medicine, University of Porto, Portugal
2   i3S, Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal
,
Raquel Soares
1   Biochemistry Unit, Department of Biomedicine, Faculty of Medicine, University of Porto, Portugal
2   i3S, Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal
,
Carla Luís
1   Biochemistry Unit, Department of Biomedicine, Faculty of Medicine, University of Porto, Portugal
2   i3S, Instituto de Investigação e Inovação em Saúde, University of Porto, Porto, Portugal
› Author Affiliations Funding This project was partially supported by Fundação para a Ciência e Tecnologia FCT (REF UID / BIM / 04293/2020) and by a scholarship assigned to Carla Luís (SFRH/BD/146489/2019).
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Abstract

Background In type 2 diabetes, insulin resistance is observed, and β-cells are incapable of responding to glycemia demands, leading to hyperglycemia. Although the nature of β-cells dysfunction in this disease is not fully understood, a link between the induction of pancreatic β-cell premature senescence and its metabolic implications has been proposed. This study aimed to understand the relationship between diabetes and pancreatic senescence, particularly at the beginning of the disease.

Methods C57Bl/6 J mice were fed two different diets, a normal diet and a high-fat diet, for 16 weeks. Pancreatic histomorphology analysis, insulin quantification, inflammation parameters, and senescence biomarkers for the experimental animals were assessed at weeks 12 and 16.

Results The results proved that diabetes onset occurred at week 16 in the High Fat Diet group, supported by glycaemia, weight and blood lipid levels. Increased β-cells size and number accompanied by increased insulin expression were observed. Also, an inflammatory status of the diabetic group was noted by increased levels of systemic IL-1β and increased pancreatic fibrosis. Finally, the expression of galactosidase-beta 1 (GLB1) was significantly increased in pancreatic β-cells.

Conclusion The study findings indicate that senescence, as revealed by an increase in GLB1 expression, is a key factor in the initial stage of diabetes.

Key words

Diabetes- pancreatic cells, cellular senescence, histomorphology, markers


Publication History

Received: 26 November 2022
Received: 11 February 2023

Accepted: 16 February 2023

Article published online:
18 April 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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