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DOI: 10.1055/a-2052-6710
Different Course of Immune Reactions and Endothelial Cell Loss after Penetrating Low-Risk Keratoplasty and Descemet Membrane Endothelial Keratoplasty for Fuchs Endothelial Dystrophy
Article in several languages: deutsch | EnglishAbstract
Background The aim of this study was to compare the incidence of immune reactions and endothelial cell loss after penetrating keratoplasty (PKP) vs. Descemet membrane endothelial keratoplasty (DMEK) in patients with Fuchs endothelial dystrophy (FED).
Patients and Methods In the present retrospective study, a total of 962 surgeries (225 excimer laser PKP and 727 DMEK) of 700 patients performed between 28.06.2007 and 27.08.2020 in the Department of Ophthalmology at Saarland University Medical Center UKS were statistically evaluated. On the one hand, the prevalence and the temporal course of the immune reactions that occurred were analysed using the Kaplan-Meier method, as well as the effect of the immune reactions on the endothelial cells and corneal thickness. Secondly, endothelial cell density, pleomorphism, and polymegethism of the endothelial cells were evaluated for the time points U1 = preoperative, U2 = 6 weeks postoperative, U3 = 6 to 9 months postoperative, U4 = 1 to 2 years postoperative, and U5 = 5 years postoperative. In addition, statistical tests were carried out for differences between the two types of surgery and in the longitudinal course.
Results A total of 54 immune reactions occurred during the observed period, whereby the probability of such a reaction was significantly greater in the PKP group with 8.9% than in the DMEK group with 4.5% (p = 0.011). The comparison of the two Kaplan-Meier curves also showed a significant difference between the two surgical techniques in the log-rank test (p = 0.012). The endothelial cell loss due to the immune reaction was only significant in PKP (p = 0.003). For all surgical procedures, endothelial cell density decreased significantly with time in both surgical techniques (p < 0.0001 in each case), but more strongly with DMEK than with PKP (p < 0.0001). Furthermore, this cell density was significantly higher with PKP than with DMEK for the whole observation time (p < 0.0001). Polymegethism decreased significantly in the DMEK group (p < 0.0001). Pleomorphism was significantly higher, on average, in DMEK than in PKP (p < 0.0001).
Conclusion The prognosis of DMEK in patients with FED seems to be more favourable after immune reactions than that of PKP, as not only were immune reactions less frequent, but they were also milder. However, endothelial cell density was significantly higher in the PKP group during the entire follow-up.
Bereits bekannt
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Die DMEK löst die PKP in Bezug auf die Indikation FED immer mehr als OP-Technik der Wahl ab.
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Bisweilen wurden einige Vorteile der DMEK gegenüber der PKP beschrieben: Im Allgemeinen treten weniger Immunreaktionen auf und der chronische Endothelzellverlust ist geringer.
Neu beschrieben
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Immunreaktionen treten bei FED nach PKP signifikant häufiger auf als nach DMEK. Außerdem verlaufen die Abstoßungsreaktionen nach DMEK in Bezug auf den Endothelzellverlust milder.
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Die Endothelzelldichte der Gesamtkohorte sinkt ausgehend von der Spenderendothelzelldichte sowohl nach PKP als auch nach DMEK signifikant ab. In dieser Studie war die Endothelzelldichte allerdings überraschenderweise im gesamten postoperativen Beobachtungszeitraum bei der PKP signifikant höher als bei der DMEK und der Zellverlust verlief nach DMEK steiler als nach PKP.
Already known:
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DMEK is increasingly replacing PKP as the surgical technique of choice for the indication of FED.
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Some advantages of DMEK over PKP have sometimes been described: In general, fewer immune reactions occur and chronic endothelial cell loss is lower.
Newly described:
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Immune reactions occur significantly more frequently in FED after PKP than after DMEK. In addition, the rejection reactions after DMEK are milder with regard to endothelial cell loss.
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The endothelial CD of the total cohort decreases significantly from the donor endothelial CD after both PKP and DMEK. In this study, however, endothelial CD was surprisingly significantly higher with PKP than with DMEK throughout the postoperative observation period and cell loss was steeper after DMEK than after PKP.
Key words
Fuchs endothelial dystrophy - penetrating keratoplasty - Descemet membrane endothelial keratoplasty - endothelial cell density - immune reactionPublication History
Received: 27 July 2022
Accepted: 28 February 2023
Article published online:
05 May 2023
© 2023. Thieme. All rights reserved.
Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany
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