Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

Ekaterina-Michaela Tomou; Lara Bieler; Tobias Spöttl; Sebastien Couillard-Despres; Helen Skaltsa; Corinna Urmann

doi:10.1055/a-2072-2351

Planta Medica, Inhaltsverzeichnis

Planta Med 2023; 89(11): 1087-1096
DOI: 10.1055/a-2072-2351

Natural Product Chemistry and Analytical Studies

Original Papers

Metabolic Fingerprinting of Different Sideritis Taxa Infusions and Their Neurogenic Activity

Ekaterina-Michaela Tomou

¹Section of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, Athens, Greece

²Weihenstephan-Triesdorf University of Applied Sciences, Organic-Analytical Chemistry, Straubing, Germany

,

Lara Bieler

³Institute of Experimental Neuroregeneration, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, Salzburg, Austria

⁴Austrian Cluster for Tissue Regeneration, Vienna, Austria

,

Tobias Spöttl

⁵TUM Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany

,

Sebastien Couillard-Despres

³Institute of Experimental Neuroregeneration, Spinal Cord Injury and Tissue Regeneration Center Salzburg (SCI-TReCS), Paracelsus Medical University Salzburg, Salzburg, Austria

⁴Austrian Cluster for Tissue Regeneration, Vienna, Austria

,

Helen Skaltsa

¹Section of Pharmacognosy & Chemistry of Natural Products, Department of Pharmacy, School of Health Sciences, National and Kapodistrian University of Athens, Panepistimiopolis, Zografou, Athens, Greece

,

Corinna Urmann

²Weihenstephan-Triesdorf University of Applied Sciences, Organic-Analytical Chemistry, Straubing, Germany

⁵TUM Campus Straubing for Biotechnology and Sustainability, Technical University of Munich, Straubing, Germany

› Institutsangaben

Abstract

Over the last years, Sideritis extracts were shown to improve memory. However, their potential to promote the generation of new neurons, starting with the neuronal differentiation of neural stem cells, remains unexplored. Therefore, the present study aimed to evaluate the neurogenic effects of different Sideritis infusions in neural stem and precursor cells and their impact on cell viability. Moreover, the metabolic fingerprints were recorded using LC-DAD, LC-HRESIMS, and GC-MS. The neurogenic potential of infusions of the eight Sideritis taxa tested was as potent as the classical neuronal inducer combination of retinoic acid and valproic acid. Further cytotoxicity assays revealed that the IC₅₀ values of the extracts were between 163 and 322 µg/mL. Hierarchical cluster analyses of the metabolic fingerprints unveiled that the two Sideritis taxa with the lowest IC₅₀ values were the most divergent in the analytical techniques used. As the analysis focused on polyphenols, it is reasonable to assume that these compounds are responsible for the effect on the cell viability of SH-SY5Y neuroblastoma cells. This study is the first report on the neurogenic potential of Sideritis taxa and might support the use of Sideritis herbal preparations in the context of neurodegenerative diseases.

Key words

Sideritis - Lamiaceae - neurogenesis - doublecortin - infusions - metabolic fingerprint

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Referenzen

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