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Horm Metab Res 2023; 55(06): 432-438
DOI: 10.1055/a-2085-6171
Original Article: Endocrine Research

Study on the Regulation of Trophoblast Activity by Abnormally Expressed lncRNA CCDC144NL-AS1 in Patients with Gestational Diabetes Mellitus

Dan Cao
1   Obstetrics and Gynecology, Changzhou No. 2 People’s Hospital, Changzhou, China
,
Yu Wang
1   Obstetrics and Gynecology, Changzhou No. 2 People’s Hospital, Changzhou, China
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Abstract

Gestational diabetes mellitus (GDM) is a common complication in pregnant women. The growth and differentiation of trophoblast cells determine the function of the placenta, and therefore further affect the transport of nutrients to the fetus. lncRNA Coiled-Coil Domain Containing 144 N-Terminal-Like antisense1 (CCDC144NL-AS1) was reported to be abnormally expressed in GDM, but its function and mechanism remain undefined. This study aimed to reveal the expression of CCDC144NL-AS1 in GDM and evaluate its significance in disease development. The expression of CCDC144NL-AS1 in serum and placenta tissues of GDM patients and healthy pregnant women was evaluated using PCR. The effect of CCDC144NL-AS1 on the proliferation, migration, and invasion of trophoblast cells was evaluated with CCK8 and Transwell assay. The mechanism of the interaction between CCDC144NL-AS1 and miR-143–3p was assessed by luciferase reporter assay and cell transfection. CCDC144NL-AS1 was upregulated in GDM patients, which discriminated GDM patients from healthy pregnant women with high sensitivity and specificity and was positively correlated with the insulin resistance indexes. In trophoblast cells, high glucose exposure induced increased CCDC144NL-AS1 and suppressed cell proliferation, migration, and invasion. Silencing CCDC144NL-AS1 could alleviate the inhibitory effect of high glucose, while the knockdown of miR-143–3p reversed the effect of CCDC144NL-AS1. In conclusion, upregulated CCDC144NL-AS1 served as a diagnostic biomarker of GDM and regulated the development of trophoblast cells via negatively modulating miR-143–3p.

Key words

lncRNA CCDC144NL-AS1 - trophoblast cell - diagnosis - development - gestational diabetes mellitus 


Publication History

Received: 05 January 2023

Accepted after revision: 25 April 2023

Article published online:
09 June 2023

© 2023. Thieme. All rights reserved.

Georg Thieme Verlag
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

  • References

  • 1 Ma RCW.. Epidemiology of diabetes and diabetic complications in China. Diabetologia 2018; 61: 1249-1260
    CrossrefPubMedSearch in Google Scholar
  • 2 Johns EC, Denison FC, Norman JE. et al. Gestational diabetes mellitus: mechanisms, treatment, and complications. Trends Endocrinol Metab 2018; 29: 743-754
    CrossrefPubMedSearch in Google Scholar
  • 3 Alejandro EU, Mamerto TP, Chung G. et al. Gestational diabetes mellitus: a harbinger of the vicious cycle of diabetes. Int J Mol Sci 2020; 21: 5003
    CrossrefPubMedSearch in Google Scholar
  • 4 Juan J, Yang H.. Prevalence, Prevention, and lifestyle intervention of gestational diabetes mellitus in China. Int J Environ Res Public Health 2020; 17: 9517
    CrossrefPubMedSearch in Google Scholar
  • 5 Silva-Zolezzi I, Samuel TM, Spieldenner J.. Maternal nutrition: opportunities in the prevention of gestational diabetes. Nutr Rev 2017; 75: 32-50
    CrossrefPubMedSearch in Google Scholar
  • 6 Sert UY, Ozgu-Erdinc AS.. Gestational diabetes mellitus screening and diagnosis. Adv Exp Med Biol 2021; 1307: 231-255
    CrossrefPubMedSearch in Google Scholar
  • 7 Dluski DF, Wolinska E, Skrzypczak M.. Epigenetic changes in gestational diabetes mellitus. Int J Mol Sci 2021; 22: 7649
    CrossrefPubMedSearch in Google Scholar
  • 8 Zhang Y, Qu L, Ni H. et al. Expression and function of lncRNA MALAT1 in gestational diabetes mellitus. Adv Clin Exp Med 2020; 29: 903-910
    CrossrefPubMedSearch in Google Scholar
  • 9 Li T, Hu D, Gong Y.. Identification of potential lncRNAs and co-expressed mRNAs in gestational diabetes mellitus by RNA sequencing. J Matern Fetal Neonatal Med 2021; 35: 5125-5139
    CrossrefPubMedSearch in Google Scholar
  • 10 Zhang Y, Zhang H, Wu S.. LncRNA-CCDC144NL-AS1 Promotes the development of hepatocellular carcinoma by inducing WDR5 expression via sponging miR-940. J Hepatocell Carcinoma 2021; 8: 333-348
    CrossrefPubMedSearch in Google Scholar
  • 11 Zhang L, Chi B, Chai J. et al. LncRNA CCDC144NL-AS1 Serves as a prognosis biomarker for non-small cell lung cancer and promotes cellular function by targeting miR-490-3p. Mol Biotechnol 2021; 63: 933-940
    CrossrefPubMedSearch in Google Scholar
  • 12 Zhang C, Wu W, Zhu H. et al. Knockdown of long noncoding RNA CCDC144NL-AS1 attenuates migration and invasion phenotypes in endometrial stromal cells from endometriosis†. Biol Reprod 2019; 100: 939-949
    CrossrefPubMedSearch in Google Scholar
  • 13 Wang Y, Guo B, Xiao Z. et al. Long noncoding RNA CCDC144NL-AS1 knockdown induces naïve-like state conversion of human pluripotent stem cells. Stem Cell Res Ther 2019; 10: 220
    CrossrefPubMedSearch in Google Scholar
  • 14 He J, Guan J, Liao S. et al. Long Noncoding RNA CCDC144NL-AS1 promotes the oncogenicity of osteosarcoma by acting as a molecular sponge for microRNA-490-3p and thereby increasing HMGA2 expression. Onco Targets Ther 2021; 14: 1-13
    CrossrefPubMedSearch in Google Scholar
  • 15 Vitiello D, Patrizio P.. Implantation and early embryonic development: implications for pregnancy. Semin Perinatol 2007; 31: 204-207
    CrossrefPubMedSearch in Google Scholar
  • 16 Assaf-Balut C, García de la Torre N, Calle-Pascual AL. et al. Detection, treatment and prevention programs for gestational diabetes mellitus: The St Carlos experience. Endocrinol Diabetes Nutr (Engl Ed) 2020; 67: 342-350
    PubMedSearch in Google Scholar
  • 17 Anderlová K. Screening for gestational diabetes mellitus. Vnitr Lek 2021; 67: 366-367
    CrossrefPubMedSearch in Google Scholar
  • 18 Tieu J, McPhee AJ, Crowther CA. et al. Screening for gestational diabetes mellitus based on different risk profiles and settings for improving maternal and infant health. Cochrane Database Syst Rev 2017; 8: Cd007222
    PubMedSearch in Google Scholar
  • 19 Filardi T, Catanzaro G, Mardente S. et al. Non-coding RNA: role in gestational diabetes pathophysiology and complications. Int J Mol Sci 2020; 21: 4020
    CrossrefPubMedSearch in Google Scholar
  • 20 Li Y, Xu K, Xu K. et al. Roles of identified long noncoding RNA in diabetic nephropathy. J Diabetes Res 2019; 5383010
    PubMedSearch in Google Scholar
  • 21 Li YX, Long DL, Liu J. et al. Gestational diabetes mellitus in women increased the risk of neonatal infection via inflammation and autophagy in the placenta. Medicine (Baltimore) 2020; 99: e22152
    CrossrefPubMedSearch in Google Scholar
  • 22 Knöfler M, Haider S, Saleh L. et al. Human placenta and trophoblast development: key molecular mechanisms and model systems. Cell Mol Life Sci 2019; 76: 3479-3496
    CrossrefPubMedSearch in Google Scholar
  • 23 Carrasco-Wong I, Moller A, Giachini FR. et al. Placental structure in gestational diabetes mellitus. Biochim Biophys Acta Mol Basis Dis 2020; 1866: 165535
    CrossrefPubMedSearch in Google Scholar
  • 24 Graham CH, Hawley TS, Hawley RG. et al. Establishment and characterization of first trimester human trophoblast cells with extended lifespan. Exp Cell Res 1993; 206: 204-211
    CrossrefPubMedSearch in Google Scholar
  • 25 Southern PJ, Berg P. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. J Mol Appl Genet 1982; 1: 327-341
    PubMedSearch in Google Scholar
  • 26 Aplin JD. Developmental cell biology of human villous trophoblast: current research problems. Int J Dev Biol 2010; 54: 323-329
    CrossrefPubMedSearch in Google Scholar
  • 27 Fan H, Ge Y, Ma X. et al. Long non-coding RNA CCDC144NL-AS1 sponges miR-143-3p and regulates MAP3K7 by acting as a competing endogenous RNA in gastric cancer. Cell Death Dis 2020; 11: 521
    CrossrefPubMedSearch in Google Scholar
  • 28 Muralimanoharan S, Maloyan A, Myatt L.. Mitochondrial function and glucose metabolism in the placenta with gestational diabetes mellitus: role of miR-143. Clin Sci (Lond) 2016; 130: 931-941
    CrossrefPubMedSearch in Google Scholar
  • 29 Li B, Fan J, Chen N.. A novel regulator of type II diabetes: MicroRNA-143. Trends Endocrinol Metab 2018; 29: 380-388
    CrossrefPubMedSearch in Google Scholar
  • 30 Li L, Zuo H, Huang X. et al. Bone marrow macrophage-derived exosomal miR-143-5p contributes to insulin resistance in hepatocytes by repressing MKP5. Cell Prolif 2021; 54: e13140
    CrossrefPubMedSearch in Google Scholar
  • 31 Bai S, Xiong X, Tang B. et al. Exosomal circ_DLGAP4 promotes diabetic kidney disease progression by sponging miR-143 and targeting ERBB3/NF-kappaB/MMP-2 axis. Cell Death Dis 2020; 11: 1008
    CrossrefPubMedSearch in Google Scholar