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CC BY 4.0 · Exp Clin Endocrinol Diabetes 2023; 131(09): 456-462
DOI: 10.1055/a-2096-9641
Article

Prevalence of Thyroid Peroxidase and Thyroglobulin Autoantibodies in the Swedish Population

Neele Bergemann
1   Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden
,
Berglind Jonsdottir
1   Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden
,
Anna-Lena Nilsson
1   Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden
,
Mikael Lantz
2   Department of Endocrinology, Department of Clinical Sciences, Skåne University Hospital, Lund University, Malmö and Lund, Sweden
,
Alexander Lind
1   Department of Clinical Sciences, Malmö, Lund University, Malmö, Sweden
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Abstract

Autoimmune thyroid disease (AITD) may be detected prior to clinical symptoms through the presence of autoantibodies against thyroid peroxidase (TPOab), thyroglobulin (TGab), or both.

The present study aimed to develop a novel radiobinding assay (RBA) for TPOab and to determine the prevalence of TPOab and TGab in the Swedish population.

Patient samples from 27 newly diagnosed Graves’ disease patients in longitudinal follow-up and 124 AITD autoantibody-positive children in prospective follow-up for increased risk of type 1 diabetes were included to validate the novel RBA for TPO. The results of RBA were compared with those obtained by commercial radioimmunoassay (RIA) and electrochemiluminescence (ECL). Furthermore, 476 serum samples from adult blood donors and 297 from 13-year-old school children were analyzed for the presence of TPOab and TGab.

Receiver operating characteristics analysis for the novel TPOab resulted in an area under curve (AUC) value of 0.82 (p<0.0001), a sensitivity of 77.8%, and a specificity of 91.9% in adult blood donors, and an AUC value of 0.70 (p<0.0001), a sensitivity of 53.2% and a specificity of 95.3% in the 13-year-old school children, respectively. TPOab levels in RBA correlated with both ECL (r=0.8950, p<0.0001) and RIA (r=0.9295, p<0.0001). The prevalence of TPOab and TGab was 6.3% and 7.6% in adult blood donors and 2.9 and 3.7% in 13-year-old school children.

In conclusion, a novel RBA for the determination of TPOab was developed and validated with current methodologies. This study also reports an increasing prevalence of thyroid autoantibodies from adolescence to adulthood.

Key words

Antibodies  - Autoimmunity - Thyroid Autoimmunity


Publication History

Received: 04 December 2022
Received: 21 February 2023

Accepted: 27 March 2023

Article published online:
28 June 2023

© 2023. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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