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Int J Sports Med 2024; 45(01): 33-40
DOI: 10.1055/a-2172-8171
Physiology & Biochemistry

CircRNA Larp4b/miR-298-5p/Mef2c Regulates Cardiac Hypertrophy Induced by Angiotensin II

Qihai Xie
1   Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, China
2   Department of Cardiology, Baoshan Branch, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China
,
Xiangdong Xu
3   Department of Cardiology, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China
,
Danqun Xiong
3   Department of Cardiology, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China
,
Man Yao
3   Department of Cardiology, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China
,
Yafeng Zhou
1   Department of Cardiology, the First Affiliated Hospital of Soochow University, Suzhou, China
› Institutsangaben Funding Information Jiangsu Province’s 333 High-Level Talents Project — BRA2017539 National Natural Science Foundation of China — http://dx.doi.org/10.13039/501100001809; 81873486, 81770327 Jiangsu Province’s Key Provincial Talents Program — ZDRCA2016043 Natural Scientific Fund of Jiangsu Province — BK20161226
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Abstract

Cardiac hypertrophy (CH) is an early marker in the clinical course of heart failure. Circular RNAs (circRNAs) play important roles in human disease. However, the role of circ_Larp4b in myocardial hypertrophy has not been studied. Angiotensin II (Ang II) treated HL-1 cells to induce a CH cell model. Quantitative real-time polymerase chain reaction was used to detect the expression of circ_Larp4b, microRNA-298-5p, and myocyte enhancer factor 2 (Mef2c). Western blot detected the protein level of alpha-actinin-2 (ACTN2), beta-myosin heavy chain (β-MHC), atrial natriuretic peptide (ANP), and Mef2c. The relationship between miR-298-5p and circ_Larp4b or Mef2c was verified by dual-luciferase reporter assay and RNA pull-down assay. Circ_Larp4b and Mef2c were upregulated in HL-1 cells treated with Ang II. Moreover, circ_Larp4b down-regulation regulated the progress of CH induced by Ang II. MiR-298-5p was a target of circ_Larp4b, and Mef2c was a target of miR-298-5p. Overexpressed Mef2c reversed the cell size inhibited by miR-298-5p in Ang II-induced HL-1 cells. Circ_Larp4b regulated CH progress by regulating miR-298-5p/Mef2c axis.

Key words

circ_larp4b - mir-298-5p - cardiac hypertrophy - mef2c - skeletal muscle - regeneration - exercise physiology - oxidative stress - myosin

Supplementary Material



Publikationsverlauf

Eingereicht: 08. November 2022

Angenommen: 22. August 2023

Artikel online veröffentlicht:
13. November 2023

© 2023. Thieme. All rights reserved.

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