Abstract
Cardiac hypertrophy (CH) is an early marker in the clinical course of heart
failure. Circular RNAs (circRNAs) play important roles in human disease.
However, the role of circ_Larp4b in myocardial hypertrophy has not been studied.
Angiotensin II (Ang II) treated HL-1 cells to induce a CH cell model.
Quantitative real-time polymerase chain reaction was used to detect the
expression of circ_Larp4b, microRNA-298-5p, and myocyte enhancer factor 2
(Mef2c). Western blot detected the protein level of alpha-actinin-2 (ACTN2),
beta-myosin heavy chain (β-MHC), atrial natriuretic peptide (ANP), and
Mef2c. The relationship between miR-298-5p and circ_Larp4b or Mef2c was verified
by dual-luciferase reporter assay and RNA pull-down assay. Circ_Larp4b and Mef2c
were upregulated in HL-1 cells treated with Ang II. Moreover, circ_Larp4b
down-regulation regulated the progress of CH induced by Ang II. MiR-298-5p was a
target of circ_Larp4b, and Mef2c was a target of miR-298-5p. Overexpressed Mef2c
reversed the cell size inhibited by miR-298-5p in Ang II-induced HL-1 cells.
Circ_Larp4b regulated CH progress by regulating miR-298-5p/Mef2c
axis.
Key words
circ_larp4b - mir-298-5p - cardiac hypertrophy - mef2c - skeletal muscle - regeneration - exercise physiology - oxidative stress - myosin