Tapirira guianensis is Selectively Cytotoxic, Induces Apoptosis to the Glioblastoma and Decreases Tumor Growth and Angiogenesis in vivo

Ana Gabriela Silva Oliveira; Marina Andrade Rocha; Lucas Santos de Azevedo; Aline Thaynara de Moura Coelho; Rafael César Russo Chagas; Hélio Batista Santos; Ralph Gruppi Thomé; Peter Samuel; Evelyn Wolfram; Bonglee Kim; Rui Manuel Reis; Rosy Iara Maciel Azambuja Ribeiro

doi:10.1055/a-2181-2569

Planta Medica, Table of Contents

Planta Med 2024; 90(01): 13-24
DOI: 10.1055/a-2181-2569

Biological and Pharmacological Activity

Original Papers

Tapirira guianensis is Selectively Cytotoxic, Induces Apoptosis to the Glioblastoma and Decreases Tumor Growth and Angiogenesis in vivo

Ana Gabriela Silva Oliveira

¹Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

,

Marina Andrade Rocha

¹Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

,

Lucas Santos de Azevedo

¹Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

,

Aline Thaynara de Moura Coelho

¹Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

,

Rafael César Russo Chagas

¹Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

,

Hélio Batista Santos

²Tissue Processing Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

,

Ralph Gruppi Thomé

²Tissue Processing Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

,

Peter Samuel

³Zurich University of Applied Sciences, Department of Life Sciences and Facility Management, Wädenswil, Switzerland

,

Evelyn Wolfram

³Zurich University of Applied Sciences, Department of Life Sciences and Facility Management, Wädenswil, Switzerland

,

Bonglee Kim

⁴Department of Pathology, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea

,

Rui Manuel Reis

⁵Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil

⁶Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Portugal

,

Rosy Iara Maciel Azambuja Ribeiro

¹Experimental Pathology Laboratory, Midwest Campus, Federal University of São João del-Rei, Divinópolis, Brazil

› Author Affiliations

Abstract

Glioblastoma is the most frequent primary malignant brain tumor without effective treatment, which makes this work extremely relevant. The study of the bioactive compounds from medicinal plants plays an important role in the discovery of new drugs.

This research investigated the constituents of Tapirira guianensis and its antitumor potential (in vitro and in vivo) in glioblastoma. The T. guianensis extracts were characterized by mass spectrometry. The ethyl acetate partition (01ID) and its fractions 01ID-F2 and 01ID-F4 from T. guianensis showed potential antitumor treatment evidenced by selective cytotoxicity for GAMG with IC50 14.1 µg/mL, 83.07 µg/mL, 59.27 µg/mL and U251 with IC50 25.92 µg/mL, 37.3 µg/mL and 18.84 µg/mL. Fractions 01ID-F2 and 01ID-F4 were 10 times more selective when compared to TMZ and 01ID for the two evaluated cell lines. T. guianensis also reduced matrix metalloproteinases 2 – 01ID-F2 (21.84%), 01ID-F4 (29.6%) and 9 – 01ID-F4 (73.42%), ID-F4 (53.84%) activities, and induced apoptosis mainly through the extrinsic pathway. Furthermore, all treatments significantly reduced tumor size (01ID p < 0,01, 01ID-F2 p < 0,01 and 01ID-F4 p < 0,0001) and caused blood vessels to shrink in vivo. The present findings highlight that T. guianensis exhibits considerable antitumor potential in preclinical studies of glioblastoma. This ability may be related to the phenolic compounds and sesquiterpene derivatives identified in the extracts. This study deserves further in vivo research, followed by clinical investigation.

Key words

Glioblastoma - cerrado - Tapirira guianensis - Anacardiaceae - apoptosis - angiogenesis

Full Text

References

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