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Planta Med 2024; 90(05): 380-387
DOI: 10.1055/a-2244-8855
Biological and Pharmacological Activity
Original Papers

Affinin, Isolated from Heliopsis longipes, Induces an Antihypertensive Effect That Involves CB1 Cannabinoid Receptors and TRPA1 and TRPV1 Channel Activation

Beatriz A. Luz-Martínez
1   Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, México
,
Dailenys Marrero-Morfa
1   Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Querétaro, México
,
Francisco J. Luna-Vázquez
2   Laboratorio de Investigación Química y Farmacológica de Productos Naturales, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Querétaro, México
,
Alejandra Rojas-Molina
2   Laboratorio de Investigación Química y Farmacológica de Productos Naturales, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Querétaro, México
,
César Ibarra-Alvarado 
2   Laboratorio de Investigación Química y Farmacológica de Productos Naturales, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, Querétaro, México
› Author AffiliationsBeatriz Adriana Luz-Martínez acknowledges Consejo Nacional de Ciencia y Tecnología (CONACYT) for his doctoral grant. The authors are very grateful to the Autonomous University of Querétaro for the funding granted through the project Química Somos Todos 2023 (FQU202312).
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Abstract

In previous studies, we demonstrated that the ethanolic extract of Heliopsis longipes roots and its main alkamide, affinin, elicit a vasorelaxant effect through a mechanism involving activation of the gasotransmitter pathways and stimulation of cannabinoid type 1 receptors and transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1 channels. However, it has not yet been demonstrated whether the EEH and affinin are capable of lowering high blood pressure. Therefore, the aim of the present study was to determine the effect of the oral administration of the EEH and affinin on the systolic blood pressure of NG-nitro-L-arginine methyl ester-induced hypertensive rats and to explore the participation of cannabinoid receptors and transient receptor potential channels in the mechanism of action of this alkamide. Our results showed that the ethanolic extract of H. longipes and affinin significantly lowered systolic blood pressure and induced an improvement in endothelial function, which is associated with increased serum nitric oxide levels. Inhibition of cannabinoid type 1 receptors by rimonabant (3 mg/kg), transient receptor potential ankyrin 1 channels by HC-030031 (8 mg/kg), and transient receptor potential vanilloid 1 channels by capsazepine (5 mg/kg) significantly decreased the antihypertensive effect induced by affinin, suggesting that the blood pressure-lowering effect of this alkamide involves activation of cannabinoid type 1 receptors and transient receptor potential ankyrin 1 and transient receptor potential vanilloid 1 channels.

Keywords

affinin - Asteraceae - CB1 cannabinoid receptor - Heliopsis longipes - hypertension - TRP channels

Supporting Information

    The effects of the oral administration of EEH and AF for 4 days on SBP are available as Supporting Information.

  • Supporting Information


Publication History

Received: 17 October 2023

Accepted after revision: 14 January 2024

Accepted Manuscript online:
14 January 2024

Article published online:
20 February 2024

© 2024. Thieme. All rights reserved.

Georg Thieme Verlag KG
Rüdigerstraße 14, 70469 Stuttgart, Germany

 

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