Protective Effects of Xanthine Derivatives Against Arsenic
                    Trioxide-Induced Oxidative Stress in Mouse Hepatic and Renal
                    Tissues

Navid Omidifar; Ahmad Gholami; Mansoureh Shokripour; Mohammad Ali Nourani; Milad Mohkam; Seyyed Mojtaba Mousavi; Seyyed Alireza Hashemi; Bagher Khorram; Amir Nili Ahmadabadi; Mahintaj Dara

doi:10.1055/a-2247-5232

Drug Research, Inhaltsverzeichnis

Drug Res (Stuttg) 2024; 74(03): 133-144
DOI: 10.1055/a-2247-5232

Original Article

Protective Effects of Xanthine Derivatives Against Arsenic Trioxide-Induced Oxidative Stress in Mouse Hepatic and Renal Tissues

Autor*innen

Navid Omidifar

¹Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

²Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Ahmad Gholami

¹Biotechnology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

³Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
Mansoureh Shokripour

²Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Mohammad Ali Nourani

²Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Milad Mohkam

⁴Allergy Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Seyyed Mojtaba Mousavi

⁵Department of Chemical Engineering, National Taiwan University of Science and Technology, Taipei City, Taiwan
Seyyed Alireza Hashemi

⁶Health Policy Research Center, Health Institute, Shiraz University of Medical Sciences, Shiraz, Iran
Bagher Khorram

⁷Student Research Committee, School of Nursing and Midwifery, Shiraz University of Medical Sciences, Shiraz, Iran
Amir Nili Ahmadabadi

⁸Department of Pharmacology and Toxicology, Medicinal Plants and Natural Products Research Center, School of Pharmacy, Hamadan University of Medical Sciences, Hamadan
Mahintaj Dara

⁹Stem Cells and Transgenic Technology Center, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract

In this study, the protective efficacy of pentoxifylline (PTX) as a xanthine derivative against arsenic trioxide (ATO)-induced kidney and liver damage in mice was investigated. Thirty-six mice were divided into six groups, receiving intraperitoneal injections of saline, ATO, PTX, or a combination for four weeks. Blood samples were analyzed for serum biochemistry, while hepatic tissue underwent examination for histopathological changes and assessment of oxidative stress markers and antioxidant gene expression through Real-Time PCR. ATO exposure significantly increased serum markers (creatinine, ALT, BUN, ALP, AST) and induced histopathological changes in the liver. Moreover, it elevated renal and hepatic nitric oxide (NO) and lipid peroxidation (LPO) levels, and reduced antioxidant enzyme expression (CAT, GSR, GPx, MPO, SOD), total thiol groups (TTGs), and total antioxidant capacity (TAC). Conversely, PTX treatment effectively lowered serum hepatic and renal markers, improved antioxidant markers, and induced histopathological alterations. Notably, PTX did not significantly affect renal and hepatic NO levels. These findings suggest that PTX offers therapeutic potential in mitigating liver and acute kidney injuries induced by various insults, including exposure to ATO.

Keywords

arsenic trioxide (ATO) - kidney - liver - oxidative damage - pentoxifylline (PTX)

Volltext

Referenzen

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