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CC BY-NC-ND 4.0 · Planta Med 2025; 91(03): 127-141
DOI: 10.1055/a-2462-4844
Original Papers

Extracts of Drynariae Rhizoma Promote Bone Formation in OVX Rats through Modulating the Gut Microbiota

Qing Lin
1   College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China
2   Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Guangzhou, Guangdong, China
,
Xinchen Ouyang
1   College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China
,
Qi Pan
1   College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China
,
Jiajia Huang
3   The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China
,
Zhifen Zhang
4   College of Pharmacy, Jinan University, Guangzhou, Guangdong, China
,
Yumei Yang
4   College of Pharmacy, Jinan University, Guangzhou, Guangdong, China
,
Haoyu Wang
1   College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China
,
Li Yang
4   College of Pharmacy, Jinan University, Guangzhou, Guangdong, China
,
Xiaofeng Zhu
1   College of Traditional Chinese Medicine, Jinan University, Guangzhou, Guangdong, China
2   Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Guangzhou, Guangdong, China
3   The First Affiliated Hospital, Jinan University, Guangzhou, Guangdong, China
,
Xiaoyun Li
4   College of Pharmacy, Jinan University, Guangzhou, Guangdong, China
,
Ronghua Zhang
2   Guangdong Provincial Key Laboratory of Traditional Chinese Medicine Informatization, Guangzhou, Guangdong, China
4   College of Pharmacy, Jinan University, Guangzhou, Guangdong, China
› InstitutsangabenThis research was funded by the National Natural Science Foundation of China (No. U24A6013, 82405121, 82274232, 82274376, and 82074287), National Key Research and Development Program of China (No. 2018YFC2 002 500), Guangdong Provincial Key laboratory of Traditional Chinese Medicine Informatization (No. 2021B1 212 040 007), Basic and Applied Basic Research Fund of Guangdong Province (2022B1 515 120 022), Fundamental Research Funds for the Central Universities (21 622 329, 21 623 115, 21 624 225), Basic and Applied Basic Research Fund of Guangzhou (SL2022A04J00516), and Construction project of Guangdong Famous Traditional Chinese Medicine Inheritance Studio of Ronghua Zhang (Guangdong Traditional Chinese Medicine Letter (2023) No. 108).
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Abstract

Drynariae Rhizoma has been commonly used as a preventive and therapeutic agent for bone diseases. However, its pharmacological mechanisms have not been fully elucidated. Here, we aimed to investigate the effects of Drynariae Rhizoma in a bilateral ovariectomized rat model and explore the correlation with gut microbiome. We established an ovariectomized rat model, which we treated with different doses of Drynariae Rhizoma (Drynariae Rhizoma-Low, 0.27 g/kg/day; Drynariae Rhizoma-Middle, 0.81 g/kg/day; Drynariae Rhizoma-High, 2.43 g/kg/day) through intragastric administration for 12 weeks. Results showed that Drynariae Rhizoma alleviated body weight, moderated bone microstructure, and promoted the expression of bone formation-related factors in ovariectomized rats, in which Drynariae Rhizoma-High showed the most significant effects among the three doses. Furthermore, the effects of Drynariae Rhizoma on promoting bone formation were correlated to the changes in microbial richness and the restorations of several genera, among which Ruminiclostridium and Ruminococcaceae_UCG_007 were positively correlated with the bone formation-related factors, and both were enriched in the Drynariae Rhizoma-High group as biomarkers. Moreover, CMP-legionaminate biosynthesis I might be a crucial pathway of Drynariae Rhizoma to regulate gut microbiota. The content of serum short-chain fatty acids in the ovariectomized rats were regulated by Drynariae Rhizoma. Our results demonstrate that Drynariae Rhizoma promotes bone formation in ovariectomized rats, and is related to the regulation of the gut microbiota structure.

Keywords

osteoporosis - Drynaria fortune - Polypodiaceae - extracts of Drynariae Rhizoma - ovariectomized rats - bone formation - gut microbiota

Supporting Information

    The flora distribution and different abundances of gut microbiota of rats at the class, order, family, and species levels are available as Supporting Information.

  • Ergänzendes Material


Publikationsverlauf

Eingereicht: 07. Mai 2024

Angenommen nach Revision: 04. November 2024

Accepted Manuscript online:
05. November 2024

Artikel online veröffentlicht:
17. Januar 2025

© 2025. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commecial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/)

Georg Thieme Verlag KG
Oswald-Hesse-Straße 50, 70469 Stuttgart, Germany

 

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