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Int J Angiol
DOI: 10.1055/a-2524-1844
Rapid Communication

Biomarkers for Postoperative Intimal Hyperplasia

Mitsuhiro Yamamura
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
,
Taichi Sakaguchi
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
,
Hiroe Tanaka
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
,
Ken-ichi Watanabe
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
,
Hisashi Uemura
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
,
Yuji Sakashita
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
,
Yoshio Teshima
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
,
Masashi Bungo
1   Department of Cardiovascular Surgery, Hyogo Medical University, Nishinomiya, Hyogo, Japan
› Author Affiliations Funding This study was supported by the Grant-in-Aid for Researchers, Hyogo College of Medicine, 2006–2007, Japan.
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Abstract

Interleukin-8 (IL-8) is a neutrophil chemotactic factor produced by vascular endothelial cells. This study aimed to evaluate whether the serum IL-8 level can be the biomarker for postoperative intimal hyperplasia in the rat model. Sixteen male Lewis rats (mean weight: 501 ± 44 g) were subjected in this study. The right epigastric vein graft is interposed into the common femoral artery with 10–0 interrupted sutures, as previously described. Vein grafts from each group were stained with hematoxylin and eosin and Elastica Verhoeff's van Gieson's at 2 and 4 weeks postoperatively. Unoperated right epigastric veins were also examined as a Sham. In Group edaravone, free-radical scavenger, edaravone (Radicut®, Mitsubishi Tanabe Pharma Corp., Osaka Japan) was administered, instead of saline. The initial areas of vein grafts were measured using computerized planimetry (NIH Image Ver. 1.61). Serum IL-8 levels were measured and compared using an unpaired t-test. This study was approved by the Hyogo College of Medicine Animal Research Committee (No. 235). The average intimal area at 4 weeks in Control group was significantly increased compared with that of Sham group (0.43 ± 0.11 vs. 0.00 ± 0.00 mm2, p < 0.01). The average serum IL-8 levels at 4 weeks were also significantly increased, compared with that of Sham group (148 ± 6 vs. 53 ± 14 pg/mL, p < 0.05). The serum IL-8 levels in Group edaravone were appeared to be suppressed but not significant (132 ± 24 pg/mL, p = 0.72). It is very important to detect the biomarker such as the serum IL-8, before the establishment of postoperative intimal hyperplasia.

Keywords

postoperative intimal hyperplasia - biomarker - interleukin-8 - free-radical scavenger - monocyte chemoattractant protein-1 - hepatocyte growth factor

Note

This study was presented at the 65th Annual World Congress, International College of Angiology in Hawaii, United States on September 25, 2024.




Publication History

Article published online:
25 February 2025

© 2025. International College of Angiology. This article is published by Thieme.

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  • References

  • 1 Grondin CM, Campeau L, Lespérance J, Enjalbert M, Bourassa MG. Comparison of late changes in internal mammary artery and saphenous vein grafts in two consecutive series of patients 10 years after operation. Circulation 1984; 70 (3 Pt 2, Suppl): I208-I212
    PubMedSearch in Google Scholar
  • 2 Yamamura M. Postoperative intimal hyperplasia: review from my research. Int J Angiol 2024; 33 (03) 135-138
    Thieme ConnectPubMedSearch in Google Scholar
  • 3 Koch AE, Polverini PJ, Kunkel SL. et al. Interleukin-8 as a macrophage-derived mediator of angiogenesis. Science 1992; 258 (5089): 1798-1801
    CrossrefPubMedSearch in Google Scholar
  • 4 Yamamura M, Miyamoto T, Hoch JR. Tumor necrosis-α expression in rat model of intimal hyperplasia. Int J Angiol 2001; 10: 77-79
    PubMedSearch in Google Scholar
  • 5 Yamamura M, Miyamoto Y, Mitsuno M. et al. Suppression of postoperative intimal hyperplasia of vein graft with edaravone in a rat model. Int J Angiol 2005; 14: 151-154
    Thieme ConnectPubMedSearch in Google Scholar
  • 6 Watanabe K, Watanabe K, Hayase T. Radical scavenging mechanism of MCI-186. Jpn Pharmacol Ther 1997; 25 (Suppl): S1699-S1707
    PubMedSearch in Google Scholar
  • 7 Yamamura M, Miyamoto Y, Mitsuno M, Tanaka H, Kobayashi Y, Ryomoto M. Suppression of postoperative intimal hyperplasia of vein grafts with edaravone in rat models - a scanning electron microscope study. Int J Angiol 2007; 16 (04) 143-145
    Thieme ConnectPubMedSearch in Google Scholar
  • 8 Yamamura M, Mitsuno M, Tanaka H, Kobayashi Y, Fukui S, Miyamoto Y. Edaravone suppresses endothelial cell injury in vein grafts of a rat model. Int J Angiol 2008; 17 (04) 221-222
    Thieme ConnectPubMedSearch in Google Scholar
  • 9 Yamamura M, Yao H, Miyamoto T. Animal model for experimental study of postoperative intimal hyperplasia. J Jpn Coll Angiol 2003; 43: 755-759
    PubMedSearch in Google Scholar
  • 10 Yamamura M, Miyamoto T, Yao H. Serum monocyte chemoattractant protein-1 levels in rat model of intimal hyperplasia. Int J Angiol 2002; 11: 80-82
    Thieme ConnectPubMedSearch in Google Scholar
  • 11 Nakamura T, Nishizawa T, Hagiya M. et al. Molecular cloning and expression of human hepatocyte growth factor. Nature 1989; 342 (6248): 440-443
    CrossrefPubMedSearch in Google Scholar
  • 12 Van Belle E, Witzenbichler B, Chen D. et al. Potentiated angiogenic effect of scatter factor/hepatocyte growth factor via induction of vascular endothelial growth factor: the case for paracrine amplification of angiogenesis. Circulation 1998; 97 (04) 381-390
    CrossrefPubMedSearch in Google Scholar
  • 13 Yamamura M, Ryomoto M, Sekiya N, Uemura H, Nishiyama M, Sakaguchi T. Serum hepatocyte growth factor level as the biomarker for postoperative intimal hyperplasia. J Vasc Surg 2020; 72 (supplement e) e260
    CrossrefPubMedSearch in Google Scholar