Abstract
Interleukin-8 (IL-8) is a neutrophil chemotactic factor produced by vascular endothelial cells. This study aimed to evaluate whether the serum IL-8 level can be the biomarker for postoperative intimal hyperplasia in the rat model. Sixteen male Lewis rats (mean weight: 501 ± 44 g) were subjected in this study. The right epigastric vein graft is interposed into the common femoral artery with 10–0 interrupted sutures, as previously described. Vein grafts from each group were stained with hematoxylin and eosin and Elastica Verhoeff's van Gieson's at 2 and 4 weeks postoperatively. Unoperated right epigastric veins were also examined as a Sham. In Group edaravone, free-radical scavenger, edaravone (Radicut®, Mitsubishi Tanabe Pharma Corp., Osaka Japan) was administered, instead of saline. The initial areas of vein grafts were measured using computerized planimetry (NIH Image Ver. 1.61). Serum IL-8 levels were measured and compared using an unpaired t-test. This study was approved by the Hyogo College of Medicine Animal Research Committee (No. 235). The average intimal area at 4 weeks in Control group was significantly increased compared with that of Sham group (0.43 ± 0.11 vs. 0.00 ± 0.00 mm2, p < 0.01). The average serum IL-8 levels at 4 weeks were also significantly increased, compared with that of Sham group (148 ± 6 vs. 53 ± 14 pg/mL, p < 0.05). The serum IL-8 levels in Group edaravone were appeared to be suppressed but not significant (132 ± 24 pg/mL, p = 0.72). It is very important to detect the biomarker such as the serum IL-8, before the establishment of postoperative intimal hyperplasia.
Keywords
postoperative intimal hyperplasia - biomarker - interleukin-8 - free-radical scavenger - monocyte chemoattractant protein-1 - hepatocyte growth factor
