Planta Med 2009; 75(1): 12-17
DOI: 10.1055/s-0028-1088333
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

Antiedematogenic and Free Radical Scavenging Activity of Swertiamarin Isolated from Enicostemma axillare

Jaishree Vaijanathappa1 , Shrishailappa Badami1
  • 1Department of Pharmaceutical Chemistry, J. S. S. College of Pharmacy, Ootacamund, Tamil Nadu, India
Weitere Informationen

Publikationsverlauf

Received: June 15, 2008 Revised: September 16, 2008

Accepted: September 17, 2008

Publikationsdatum:
12. November 2008 (online)

Abstract

Swertiamarin, a secoiridoid isolated from the ethyl acetate extract of Enicostemma axillare, was evaluated for antiedematogenic activity using carrageenan-, formalin-, and histamine-induced paw edema methods in rats. In the carrageenan-induced method, the percentages of edema inhibition obtained after 5 h induction were 38.60, 52.50, and 45.44, respectively, for 100 and 200 mg/kg bw swertiamarin and 100 mg/kg bw of standard diclofenac sodium given orally. The activity of swertiamarin at 200 mg/kg bw was found to be superior to that of standard diclofenac sodium in all these methods. Swertiamarin was also screened for in vitro antioxidant activity using seven different methods: good activity was observed in ABTS and hydrogen peroxide methods, and moderate activity was observed in hydroxyl radical by deoxyribose and lipid peroxidation methods, with IC50 values of 2.83, 5.70, 52.56, and 78.33 μg/mL, respectively. The total antioxidant capacity was found to be 4.51 mM of ascorbic acid per gram of swertiamarin. Swertiamarin possesses antiedematogenic and in vitro antioxidant activities, and it may be the active constituent responsible for the anti-inflammatory activity of E. axillare.

Abbreviations

ABTS:2,2′-azino-bis(3-ethylbenzo-thiazoline-6-sulfonic acid) diammonium salt

BHA:butylated hydroxy anisole

bw:body weight

CMC:carboxymethylcellulose

CPCSEA:Committee for the Purpose of Controland Supervision of Experiments on Animals

DMSO:dimethyl sulfoxide

EDTA:ethylene diamine tetra acetic acid disodium salt

HPTLC:high-performance thin-layer chromatography

H2O2:hydrogen peroxide

IC50:inhibition cocnentration 50 %

NEDD:nephthyl ethylene diamine dihydrochloride

OECD:Organisation for Economic Cooperationand Development

PBS:phosphate-buffered saline

p-NDA:p-nitroso dimethyl aniline

SOD:super oxide dismutase

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Dr. Shrishailappa Badami

Professor and Director

Sree Siddaganga College of Pharmacy

Tumkur 572 102

Karnataka

India

Telefon: +91-816-273331

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