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DOI: 10.1055/s-0028-1088341
© Georg Thieme Verlag KG Stuttgart · New York
Genotoxicity Evaluation of Three Benzopyrans from Hypericum polyanthemum
Publication History
Received: May 30, 2008
Revised: August 28, 2008
Accepted: September 25, 2008
Publication Date:
07 November 2008 (online)
![](https://www.thieme-connect.de/media/plantamedica/200901/lookinside/thumbnails/10.1055-s-0028-1088341-1.jpg)
Abstract
In the present study we used the micronuclei test and the comet assay in mice to investigate the genotoxic and mutagenic effects of three benzopyrans – 6-isobutyryl-5,7-dimethoxy-2,2-dimethyl-benzopyran (HP1); 7-hydroxy-6-isobutyryl-5-methoxy-2,2-dimethyl-benzopyran (HP2); and 5-hydroxy-6-isobutyryl-7-methoxy-2,2-dimethyl-benzopyran (HP3) – isolated from Hypericum polyanthemum. No significant difference in polychromatic erythrocyte (PCE) percentage between the vehicle group and groups treated with HP1, HP2, or HP3 was detected, indicating no toxicity to the bone marrow of the animals. Only HP1 increased the frequency of micronucleated cells (MNPCEs) in bone marrow sampled after 24 h in comparison with the vehicle group, suggesting a weak mutagenic effect. The damage index and damage frequency did not show a significant increase after treatment with HP1, HP2, or HP3 in comparison with the vehicle group. The antitumor activity previously reported in vitro for these benzopyrans, the lack of acute toxicity, the MN induction only for HP1, and the relatively low DNA damage make all compounds good candidates for in vivo studies on antitumor action.
Key words
benzopyrans - Hypericum polyanthemum - Clusiaceae - comet assay - micronuclei test
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Prof. Dr. Alexandre de Barros Falcão Ferraz
Programa de Pós-graduação em Genética e Toxicologia Aplicada
Universidade Luterana do Brazil
Av. Farroupilha
8001 Canoas RS
Brazil
Phone: +51-3477-9214
Fax: +51-3477-9239
Email: alexandre.ferraz@ulbra.br