Planta Med 2009; 75(1): 62-64
DOI: 10.1055/s-0028-1088363
Pharmacology
Letter
© Georg Thieme Verlag KG Stuttgart · New York

Antioxidant Activity of Citrus Cultivars and Chemical Composition of Citrus karna Essential Oil

Swadesh Malhotra1 , Samiksha Suri1 , Rakesh Tuli1
  • 1National Botanical Research Institute, Lucknow, India
Weitere Informationen

Publikationsverlauf

Received: May 8, 2008 Revised: September 6, 2008

Accepted: September 15, 2008

Publikationsdatum:
24. November 2008 (online)

Abstract

The genus Citrus has a number of species and hybrids that are well established for their pharmaceutical and economic importance. The essential oil from Citrus karna Raf (Rutaceae) was analyzed for d-limonene (92.31 %), the major chemical constituent, along with other minor constituents such as α-pinene (1.23 %) and β-pinene (1.80 %). It showed significant inhibition for the oxidation of linoleic acid in the β-carotene-linoleic acid system. Essential oils A and B obtained from C. sinensis, with 35.08 % and 76.68 % d-limonene, respectively, were used to evaluate the effect of the d-limonene concentration on antioxidant potential. Studies showed that d-limonene and C. karna essential oil have a similar antioxidant potential (39.6 and 38.3 %, respectively). C. sinensis oils A and B showed only 10.5 % and 30 % antioxidant potential, respectively, indicating the possible role of d-limonene in antioxidant activity.

Abbreviations

BHA:butylated hydroxyanisole

BHT:butylated hydroxytoluene

FRSA:free radical scavenging activities

RI:retention indices

References

  • 1 Asolkar L V, Kakkar K K, Chakre O L. Second supplement to glossary of Indian medicinal plants with active principles. New Delhi; National Institute of Science Communication 1992: 210-1
  • 2 Grassmann J. Terpenoids as plant antioxidants.  Vitam Horm. 2005;  72 505-35
  • 3 Burton G W, Ingold K U. Beta carotene an unusual type of lipid antioxidant.  Science. 1984;  224 569-73
  • 4 Rao B N. Bioactive phytochemicals in Indian foods and their potential in health promotion and disease prevention.  Asia Pac J Nutr Clin. 2003;  12 9-22
  • 5 Patil B, Jayaprakasha G K, Harris E D. Impact of Citrus limonoids on human health.  Acta Hort. 2007;  744 127-34
  • 6 Sun C D, Chen K, Chen Y, Chen Q J. Content and antioxidant capacity of limonin and nomilin in different tissues of Citrus fruit of four cultivars during fruit growth and maturation.  Food Chem. 2005;  93 599-605
  • 7 Anagnostopoulou M A, Kefalas P, Papageorgiou V P, Assimopoulou A N, Boskou D. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis).  Food Chem. 2006;  94 19-25
  • 8 Gorinstein S, Belloso O M, Park Y, Haruenkit R, Lojek A, Ciz M. Comparison of some biochemical characteristics of different Citrus fruits.  Food Chem. 2001;  74 309-15
  • 9 Larrauri J A, Ruperez P, Bravo L, Calixto F S. High dietary fibre powders from orange and lime peels: associated polyphenols and antioxidant capacity.  Food Res Int. 1996;  29 757-62
  • 10 Hakim I A, Harris R B, Ritenbaugh C. Citrus peel use is associated with reduced risk of squamous cell carcinoma of the skin.  Nutr Cancer. 2000;  37 161-8
  • 11 Fukumoto S, Sawasaki E, Okuyama S, Miyake Y, Yokogoshi H. Flavor components of monoterpenes in Citrus essential oil.  Nutr Neurosci. 2006;  9 73-80
  • 12 Leo F D, Bosco S FD. Citrus flavonoids as bioactive compounds: Role, bioavailability, socio-economic impact and biotechnological approach for their modification. 9th International Conference on Agricultural Biotechnology: Ten Years After Ravello, Italy; 2005
  • 13 Mokbel M S, Hashinaga F. Evaluation of the antioxidant activity of extracts from buntan (Citrus grandis Osbeck) fruit tissues.  Food Chem. 2006;  94 529-34
  • 14 Jagetia G C, Venkatesha V A, Reddy T K. Naringin, a Citrus flavonone, protects against radiation-induced chromosome damage in mouse bone marrow.  Mutagenesis. 2003;  18 337-43
  • 15 Malhotra S, Banerjee R, Gupta A K, Jain S M. Seasonal variation in volatile content of Citrus sinensis. .  Indian Perfumer. 2007;  51 35-6
  • 16 Jennings W, Shibamato T. Qualitative analysis of flavor and fragrance volatiles by glass capillary gas chromatography. New York; Academic Press 1980: 9-10
  • 17 Jayaprakakasha G K, Selvi T, Sakariah K K. Antibacterial and antioxidant activities of grape (Vitis vinifera) seed extracts.  Food Res Int. 2003;  36 117-22
  • 18 Tepe B, Tepe A S, Daferera D M, Polissiou M, Sokmen A. Chemical composition and antioxidant activity of the essential oil of Clinopodium vulgare L.  Food Chem. 2007;  103 766-70
  • 19 Clevenger J. Apparatus for the determination of volatile oil.  J Am Pharm Assoc. 1928;  17 345-9
  • 20 Emmons C L, Peterson D M. Antioxidant activity and phenolic contents of oats, groats and hulls.  Cereal Chem. 1999;  76 902-5

Dr. Swadesh Malhotra

Nutraceutical & Phytochemistry Division

National Botanical Research Institute

(Council of Scientific & Industrial Research)

Rana Pratap Marg

Lucknow - 226001

UP

India

Telefon: +91-522-220-5831-35, extn 287, 354

Fax: +91-522-220-5836

eMail: malhotra54@rediffmail.com