Planta Med 2009; 75(1): 41-48
DOI: 10.1055/s-0028-1088364
Pharmacology
Original Paper
© Georg Thieme Verlag KG Stuttgart · New York

In vitro Antitumor Mechanisms of Various Scutellaria Extracts and Constituent Flavonoids

Prahlad Parajuli1 , Nirmal Joshee2 , Agnes M. Rimando3 , Sandeep Mittal1 , Anand K. Yadav2
  • 1Department of Neurosurgery, Wayne State University & Karmanos Cancer Institute, Detroit, MI, USA
  • 2Agricultural Research Station, Fort Valley State University, Fort Valley, GA, USA
  • 3USDA-ARS, Natural Products Utilization Research Unit, University, MS, USA
Weitere Informationen

Publikationsverlauf

Received: June 26, 2008 Revised: September 8, 2008

Accepted: September 12, 2008

Publikationsdatum:
24. November 2008 (online)

Abstract

Scutellaria is a traditional herbal remedy with potential anti-cancer activity. The purpose of this study was to evaluate anticancer mechanisms of thirteen Scutellaria species and analyze their leaf, stem and root extracts for levels of common biologically active flavonoids: apigenin, baicalein, baicalin, chrysin, scutellarein, and wogonin. Malignant glioma, breast carcinoma and prostate cancer cells were used to determine tumor-specific effects of Scutellaria on cell proliferation, apoptosis and cell cycle progression, via the MTT assay and flow cytometry-based apoptosis and cell cycle analysis. The extracts and individual flavonoids inhibited the proliferation of malignant glioma and breast carcinoma cells without affecting primary or non-malignant cells. The flavonoids exhibited different mechanisms of anti-tumor activity as well as positive interactions. The antitumor mechanisms involved induction of apoptosis and cell cycle arrest at G1/G2. Of the extracts tested, leaf extracts of S. angulosa, S. integrifolia, S. ocmulgee and S. scandens were found to have strong anticancer activity. This study provides basis for further mechanistic and translational studies into adjuvant therapy of malignant tumors using Scutellaria leaf tissues.

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P. Parajuli, Ph.D.

Department of Neurosurgery

Wayne State University & Karmanos Cancer Institute

4100 John R. St.

HW-CRC #607

Detroit, MI 48201

USA

Telefon: +1-313-576-8353

Fax: +1-313-576-8306

eMail: pparajuli@med.wayne.edu