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Horm Metab Res 1979; 11(8): 472-477
DOI: 10.1055/s-0028-1092764
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© Georg Thieme Verlag KG Stuttgart · New York

In Vitro Effects of Fatty Acids on the Actions of Serum on Rat and Pig Cartilage

D. F. Nutting1 , Jane C. Zollinger2 , Linda J. Coats1
  • 1Department of Physiology and Biophysics, University of Tennessee Center for the Health Sciences Memphis, Tennessee, U.S.A.
  • 2Southwestern University at Memphis, Memphis, Tennessee, U.S.A.
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Publication History

Publication Date:
17 December 2008 (online)

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Abstract

It has been reported that fatty acids preferentially inhibit serum-stimulated incorporation of sulfate by embryonic chick cartilage, suggesting that they may interfere with the effects of a proposed mediator (serum somatomedin) of the actions of growth hormone (GH). This was studied further in mammalian cartilage. Butyrate and octanoate at concentrations of 0.5 to 5 mM produced a concentration dependent inhibition of both basal and serum-stimulated sulfate and thymidine incorporation by costal cartilage from hypophysectomized rats. Butyrate also inhibited basal and serum-stimulated sulfate incorporation in cartilage from normal pigs and normal suckling rats. In all 3 test systems, oleate (0.2-5 mM) bound to serum albumin (4 g/dl) was ineffective. There was no evidence that fatty acids preferentially inhibited the stimulation of sulfate incorporation produced by serum.

Key words

Sulfation Factor (Somatomedin) - Fatty Acids - Cartilage