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Horm Metab Res 1978; 10(5): 396-400
DOI: 10.1055/s-0028-1093400
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© Georg Thieme Verlag KG Stuttgart · New York

Action of Insulin on Thyroid Protein Synthesis in Dogs

A.  Brisson-Lougarre [*] , C.  Blum [**] , Ph.  De Nayer
  • Laboratoire de Médecine Expérimentale, Faculté de Médecine-Purpan, Université Paul Sabatier, Toulouse, France and
    Institut de Pathologie Cellulaire, Université Catholique de Louvain, Bruxelles, Belgique
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Publication Date:
23 December 2008 (online)

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Abstract

The study was performed on dog thyroid slices after in vivo administration of monocomponent insulin (carotid perfusion of a lobe: 25 mIU/kg/min for 15 min, the 2nd lobe perfused as control: saline) and after in vitro hormone addition (100 mIU/ml of glucose-free incubation medium). The 3H-leucine incorporation in thyroid proteins (dpm/mg) was determined following incubation of slices for 1 h and 2 h.

Insulin given in situ significantly increased the mean incorporation into homogenate and cytosol proteins: about 130% after 1 h of incubation, for an identical hypoglycemia in both lobes. When insulin was added to the medium, a similar response was observed after 1 h of incubation, the effect being less pronounced after 2 h but still significant. The sucrose gradient analysis of the soluble fraction showed that the stimulation affected all thyroid proteins.

Polyribosomes isolated from in vivo insulin-perfused thyroids exhibited an increased activity when tested in cell-free system.

These data suggest that insulin stimulated the thyroid protein synthesis in dogs through a direct action on the thyroid cell.

Key words

Insulin - Thyroid Protein Synthesis - Dog - In Vivo Perfusion - In Vitro Addition

1 Centre National de la Recherche Scientifique (CNRS, Paris, France)

2 Institut National de la Santé et de la Recherche Médicale (INSERM, Paris, France)