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Horm Metab Res 1977; 9(5): 370-374
DOI: 10.1055/s-0028-1093530
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© Georg Thieme Verlag KG Stuttgart · New York

Heterogeneity of Extractable Gut Glucagon-Like Immunoreactivity (GLI) and Its Lipolytic Activity

K.  Horigome , A.  Ohneda , Y.  Maruhama , R.  Abe , Y.  Kai
  • The Third Medical Department, Tohoku University School of Medicine, Sendai, Japan
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Publication History

Publication Date:
23 December 2008 (online)

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Abstract

Gut glucagon-like immunoreactivity (GLI) was extracted from the mucosa of the canine intestine and was separated into two peaks by gel filtration; Peak I (7,000 daltons) and Peak II (3,500 daltons). These two peaks were purified by means of affinity chromatography using an anti-glucagon antibody as a ligand substance. These purified gut GLIs were not composed of single component, when applied to ion exchange chromatography. These partially purified Peak I and Peak II showed lipolytic activities, though very weak in potency, in vitro on the rat fat tissue at pharmacologically high concentration (5 ng eq/ml in final). It was concluded that gut GLI, even though purified, showed heterogeneity and that gut GLI revealed lipolytic activity although weak in potency compared with pancreatic glucagon.

Key words

Gut Glucagon-Like Immunoreactivity - Heterogeneity - Lipolysis