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Horm Metab Res 1977; 9(3): 223-227
DOI: 10.1055/s-0028-1093541
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© Georg Thieme Verlag KG Stuttgart · New York

Improved Method and its Clinical Application of a Radioimmunoassay of Arginine Vasopressin in Human Serum

H.  Wagner [*] , V.  Maier , H. E. Franz
  • Sektion Nephrologie und Abteilung für Endokrinologie und Stoffwechsel, Zentrum für Innere Medizin und Kinderheilkunde der Universität Ulm, Ulm (Donau) Germany
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Publication History

Publication Date:
23 December 2008 (online)

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Abstract

A sensitive and specific double-antibody radioimmunoassay for measuring circulating levels of arginine vasopressin in human serum is described. It is possible to detect arginine vasopressin levels of 1 µU/ml serum without extraction procedure. Normal subjects were found to have 5.7 ± 4.4 µU/ml after a dehydration period of 12 hours. Water loading diminished arginine vasopressin concentrations while dehydration increased it. Application of furosemide over a period of 14 days brought forth constant but not significant decreases.

Subjects suffering from psychogenic polydipsia showed normal levels in spite of drinking 8-12 liters of water per day. Patients suffering from liver cirrhosis with ascites showed significantly higher arginine vasopressin levels, approaching normal values, when ascites was under control.

Key words

Arginine Vasopressin-Radioimmunoassay - Liver Cirrhosis - Polydipsia - Diabetes Insipidus

1 Supported by the Deutsche Forschungsgemeinschaft, Bonn - Bad Godesberg, SFB 87 Ulm.