The influence of Clofibrate on the glycolytic pathway in liver was studied. The changes
in the activity of glucokinase and hexokinase were not significant. A reduction of
phosphofructokinase (p < 0.05) and pyruvate kinase activity was found (p < 0.0005)
during Clofibrate feeding. An in vitro inhibition of these enzymes could not be demonstrated
by Clofibrate up to a concentration of 2.5 mM. Crossover plots of glycolytic intermediates
indicate that the reduced pyruvate kinase activity may influence the glycolytic pathway
in vivo. Clofibrate feeding induces a lower ATP:ADP ratio, a lower adenylate energy
charge and elevates AMP levels in rat liver. This may possibly stimulate the hepatic
glycogenolysis and the glucose utilisation by this organ.
Glycolytic Pathway - Adenine Nucleotides - Rat Liver - Clofibrate
