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Horm Metab Res 1976; 8(6): 459-461
DOI: 10.1055/s-0028-1093595
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© Georg Thieme Verlag KG Stuttgart · New York

Superior Immunoreactivity of 125l-(Des-Tyr-βAla)-Secretin with Rabbit Anti-Secretin Sera Compared to 125l-Secretin and 125l-6-Tyrosyl Secretin

H. -J. Urbach , W.  Domschke , M.  Reiß , S.  Domschke , G.  Rosselin , E.  Wünsch , E.  Jaeger , L.  Moroder , L.  Demling
  • Department of Medicine, University of Erlangen-Nürnberg, Erlangen, Max Planck Institute of Biochemistry, München, Germany, and
    I.N.S.E.R.M., Saint Antoine Hospital, Paris, France
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Publication Date:
23 December 2008 (online)

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Abstract

A secretin analogue in which the normal amino acid sequence had been elongated by a (Des-Tyr-βAla)-residue was studied as tracer for secretin radioimmunoassay. 125I-(DATA)-secretin exhibited superior immunoreactivity with several rabbit anti-secretin sera compared to 125I-6-Tyr-secretin and also to secretin iodinated at its N-terminal histidyl residue. This may be due, at least in part, to higher conformational integrity of the secretin moiety in the 125I-(DATA)-secretin molecule. Thus, at present, 125l-(DATA)-secretin appears to be most suitable as tracer for sensitive secretin radioimmunoassay.

Key words

Immunoreactivity - Labeled Tracers - Radioimmunoassay - Secretin