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Horm Metab Res 1975; 7(5): 415-418
DOI: 10.1055/s-0028-1093696
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© Georg Thieme Verlag KG Stuttgart · New York

Effects of SH-Reagents of Different Molecular Size upon Glucose Metabolism in Isolated Rat Fat Cells[*]

H.  Kather , B.  Simon
  • Klinisches Institut zur Erforschung des Herzinfarktes an der Medizinischen Universitätsklinik, Heidelberg, Germany
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Publication Date:
23 December 2008 (online)

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Abstract

To study the role of membrane SH-groups in glucose transport of isolated rat fat cells we compared the effects of a small organic mercurial reagent p-CMB with those of a large p-CMB-derivative - p-CMB-Dextran, MW ∼ 10.000 -. It could be shown that both compounds were of almost identical reactivity on fat cell homogenate metabolism.

When applied to intact fat cells uncoupled p-CMB showed an (1) insulin like enhancement of 14C incorporation from (U-14C) glucose into CO2 and triglyceride, (2) inhibition of the insulin-stimulatory effect on these parameters and (3) inhibition of basal glucose uptake dependent on the concentrations used.

Identical concentrations of p-CMB-Dextran, however, failed to influence basal glucose uptake as well as the insulin mediated increase in glucose metabolism.

Key words

Isolated Fat Cells - p-CMB - p-CMB-Dextran - Membrane SH-Groups - Glucose Metabolism - Insulin Action

1 Parts of this work has been published as Short Communication in this Journal (Vol. 7: 98-99, 1975)

1 Parts of this work has been published as Short Communication in this Journal (Vol. 7: 98-99, 1975)

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