Subscribe to RSS
DOI: 10.1055/s-0028-1093780
© Georg Thieme Verlag KG Stuttgart · New York
Streptozotocin-Diabetes in the Chinese Hamster
Volumetric Quantitation of the Pancreatic Islets and Inhibition of Diabetes with Nicotinamide[*]Publication History
Publication Date:
23 December 2008 (online)
Abstract
A relationship was found between the dose of intraperitoneally injected streptozotocin and the degree of blood glucose elevation. At a dose of 200 mg streptozotocin/kg body weight administered intraperitoneally in Chinese hamsters, about 50% of the injected animals died within an experimental period of 3 months in a diabetic state with hyperglycemia and glucosuria. The diabetic animals showed a biphasic blood glucose curve; there was a marked hyperglycemic phase one day after injection, followed by a decrease in blood glucose to moderately elevated values which persisted to the end of the experimental period with no signs of returning to normal.
Marked islet atrophy (p <0.001) and a decrease in mean islet diameter (p<0.05) was found in the diabetic animals as compared with normal controls one month after injection.
Pretreatment with 500 mg nicotinamide/kg body weight intraperitoneally 10 minutes before the injection of streptozotocin completely inhibited the hyperglycemic effect of the latter drug.
The results show that in the Chinese hamster injection of streptozotocin causes a permanent diabetic state different from alloxan diabetes, with no signs of restitution, similar to spontaneously occurring hereditary diabetes in Chinese hamsters and human diabetes mellitus.
Key words
Chinese Hamster - Streptozotocin - Nicotinamide - Hyperglycemia - Pancreatic Islet Volume and Diameter
1 Supported by grants from the Swedish Medical Research Council (Project No. B72-12X-718-07A) and the Swedish Diabetes Foundation.
1 Supported by grants from the Swedish Medical Research Council (Project No. B72-12X-718-07A) and the Swedish Diabetes Foundation.