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Horm Metab Res 1974; 6(4): 300-303
DOI: 10.1055/s-0028-1093833
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© Georg Thieme Verlag KG Stuttgart · New York

A Rapid Procedure for the Radioimmunoassay of Rat Growth Hormone[*]

F.  Hervás , G.  Morreale de Escobar
  • Departamento de Endocrinología Experimental, Instituto G. Marañón, Centro de Investigaciones Biológicas, C.S.I.C., Velázquez 144, Madrid (Spain)
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Publication History

Publication Date:
08 January 2009 (online)

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Abstract

The radioimmunoassay for rat GH may be improved and the time required for its completion shortened by adding the labeled GH 6 hr after onset of a 24 hr incubation period at 37°C. Several features are also described which decrease the time spent on a given assay, and results are shown which validate the shortening procedures.

Key words

Radioimmunoassay - Growth Hormone - Rat - Shortened Procedure of Rat GH-RIA

1 This work has been carried out under research grant 540-11 from the Fondo Nacional para el Desarrollo de la Investigación Científica of Spain.

1 This work has been carried out under research grant 540-11 from the Fondo Nacional para el Desarrollo de la Investigación Científica of Spain.