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Horm Metab Res 1974; 6(1): 53-57
DOI: 10.1055/s-0028-1093884
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© Georg Thieme Verlag KG Stuttgart · New York

Effect of Cyclic AMP on Ketogenesis in Liver Homogenates from Pancreatectomized-Hypophysectomized Dogs[*]

A. G. Roldán [**] , E. J. del Castillo [***] , V. G. Foglia [**]
  • Instituto de Biología y Medicina Experimental, Obligado 2490, Buenos Aires, Argentina
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Publication Date:
07 January 2009 (online)

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Abstract

Liver homogenates of normal (N), pancreatectomized (Pp) and pancreatectomized-hypophysectomized (PpHp) dogs were incubated for 60 minutes and ketone body production was determined. It was found that at all times ketone body concentration in the medium was higher in Pp dogs than in normal ones and lower in PpHp dogs. Addition of 10-3 M of cAMP to the incubation medium increased ketone body formation of liver homogenates from normal dogs 50% while liver from PpHp dogs increased 164%. When liver slices from diabetic dogs were incubated with 10-3 M of propranolol a significant decrease in ketone body production was observed. The experiments described here are further evidence that hypophysectomy prevents ketosis in diabetic animals not only by diminishing FFA release from adipose tissue but also by decreasing lipolysis from the triglycerides of the liver and consequently lowering FFA release and its oxidation to ketone bodies probably by diminishing cAMP levels.

Key words

Ketone Bodies - Hypophysectomy - Pancreatectomy - cAMP - Propranolol - Liver Triglycerides

1 This work was carried out under the partial support of the University of Buenos Aires.

1 This work was carried out under the partial support of the University of Buenos Aires.

2 Members of "Carrera del Investigador", Consejo Nacional de Investigaciones Cientificas y Técnicas de la República Argentina.

3 Grantee, Ministerio de Bienestar Social de la Provincia de Buenos Aires.