Immunofluorescent Identification of Cells Synthesizing Human Growth Hormone in Culture

T. J. Martin; C. Beck; E. Guli; H. G. Burger; P. B. Greenberg; R. A. Melick

doi:10.1055/s-0028-1094132

Hormone and Metabolic Research, Inhaltsverzeichnis

Horm Metab Res 1971; 3(5): 309-313
DOI: 10.1055/s-0028-1094132

Originals

Immunofluorescent Identification of Cells Synthesizing Human Growth Hormone in Culture[*]

T. J. Martin , C. Beck , E. Guli , H. G. Burger , P. B. Greenberg , R. A. Melick

Department of Medicine, University of Melbourne,
Department of Pathology, Monash University and
Medical Research Center, Prince Henry's Hospital, Melbourne, Australia

Abstract

als PDF herunterladen

Abstract

Cells have been established in culture from human pituitary tissue obtained from an acromegalic patient. High levels of immunoreactive growth hormone were released into culture media during the first six weeks of culture. Cells transformed with SV40 virus continued to produce hormone after several subcultures over eight months. The cells synthesizing growth hormone were identified by immunofluorescence. Evidence is presented that the cells have incorporated ¹⁴C-leucine into growth hormone synthesized and released into the medium. The growth hormone appears to be secreted in a monomeric form.

Key words

Human Growth Hormone - Acromegaly - Immunoassay of Human Growth Hormone - Growth Hormone Secretion

PDF (328 kb)

Referenzen

1 This work was supported by the National Health and Medical Research Council of Australia, the Victorian Anti-Cancer Council and the Joseph Hermann Trust (University of Melbourne). We are grateful to Professor R.C. Nairn for advice and to Mr. D. Quinn for excellent technical assistance.