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Horm Metab Res 1971; 3(5): 313-317
DOI: 10.1055/s-0028-1094133
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© Georg Thieme Verlag KG Stuttgart · New York

Production of Antibodies to ACTH for Use in Radioimmunoassay[*]

K. H. Voigt , H. L. Fehm , E. F. Pfeiffer
  • Department of Endocrinology and Metabolism, Center of Internal Medicine and Pediatrics, University of Ulm, Germany
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Publication History

Publication Date:
07 January 2009 (online)

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Abstract

A reliable method for the production of ACTH-antibodies with high titres is described. ACTH was conjugated by means of ethyl-carbodiimide. Guinea pigs and rabbits were immunized. The sensitivity of the resulting antisera was evaluated by plotting a standard-curve with each antiserum. With the best antisera 1 pg ACTH/ml could be measured. Specificity of the antisera was tested by setting up antiserum dilution curves with labelled αp 1-39 ACTH, βh 1-39 ACTH and β1-24 ACTH. Surprisingly, most of the antisera appeared to react specifically with the N-terminal portion of the ACTH-chain.

Key words

Antibodies to ACTH - Sensitivity of Antibodies - Specificity of Antibodies - Radioimmunoassay of ACTH

1 Supported by Deutsche Forschungsgemeinschaft, Bad Godesberg (SFB 87/Proj. 18).

1 Supported by Deutsche Forschungsgemeinschaft, Bad Godesberg (SFB 87/Proj. 18).