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Horm Metab Res 1971; 3(5): 336-340
DOI: 10.1055/s-0028-1094138
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© Georg Thieme Verlag KG Stuttgart · New York

A Rapid Filter Paper Disk Assay for Picomole Amounts of Cyclic AMP Using a Cyclic AMP Dependent Protein Kinase[*]

F. R. Butcher [**]
  • University of Wisconsin, McArdle Laboratory for Cancer Research, Madison, Wisconsin, USA
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Publikationsdatum:
07. Januar 2009 (online)

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Abstract

A rapid filter paper disk assay for cyclic AMP based on the activation of a cyclic AMP dependent protein kinase from rabbit skeletal muscle is described. This procedure permits determination of as little as 0.2 picomoles of cyclic AMP. In addition this procedure employs ATP-γ-33P instead of ATP- γ-32P.

Key words

Cyclic AMP - Cyclic AMP Assay - Protein Kinase

1 This work was performed while the author was a USPHS postdoctoral fellow in the laboratory of Dr. Van R. Potter. Financial support was provided by departmental Grant CA 07175, Training Grant T01-Ca5002, and a USPHS postdoctoral fellowship 5 F02-CA-43,880-02 through the National Cancer Institute.

1 This work was performed while the author was a USPHS postdoctoral fellow in the laboratory of Dr. Van R. Potter. Financial support was provided by departmental Grant CA 07175, Training Grant T01-Ca5002, and a USPHS postdoctoral fellowship 5 F02-CA-43,880-02 through the National Cancer Institute.

2 Address after July 1: Brown University; Division of Biological and Medical Sciences; Providence, Rhode Island 02912