Metabolism of Steroid Conjugates - VI. In Vivo Perfusion of the Human Liver with Synthetic 7α-³ H-Dehydroepiandrosterone Sulfatide[*]

Abstract

In the course of the in vivo perfusion of the human liver with synthetic 7 α-³H-DHEA sulfatide, it could be demonstrated that up to 12% of ³H-activity in hepatic vein blood was present in the fraction of free steroids as well as in the fraction of steroid glucuronosides. With regards to the metabolism of the substrate, the metabolites of DHEA in the different fractions accounted for more than 35% of isolated ³H-activity. Besides androstenediol, 16α-OH-dehydroepiandrosterone, androstenetriol, androsterone and etiocholanolone as the predominant metabolites, significant amounts of ³H-labeled androgens and estrogens were also detected. In general, the hydrolysis and metabolism of the lipophile DHEA sulfatide greatly exceeded that found after perfusion of the human liver with DHEA sulfate.

Key words

Perfusion of the Human Liver - DHEA Sulfatide

1 Dedicated to Prof. Dr. Dr. W. Zimmermann, Institut fur Hygiene und Mikrobiologie, Universität des Saarlandes, 665 Homburg/Saar, on the occasion of his 60th birthday

1 Dedicated to Prof. Dr. Dr. W. Zimmermann, Institut fur Hygiene und Mikrobiologie, Universität des Saarlandes, 665 Homburg/Saar, on the occasion of his 60th birthday