Abstract
Insulin (0.1 U/kg) and tolbutamide (175 mg) sensitivity tests were performed in normal dogs either alone or 120 minutes after a single intravenous injection of bovine growth hormone (3 mg). No growth hormone influence upon the ability of exogenous insulin to effect blood glucose and free fatty acid removal was noted. This appears to be due to a masking of growth hormone effects by pharmacologic doses of insulin, since growth hormone did cause impairment of tolbutamide evoked endogenous insulin action upon net glucose disappearance. The injection of actinomycin D (7.5 µg/kg I.V.) 90 minutes prior to growth hormone normalized the metabolic responses to tolbutamide. In addition, the ability of growth hormone to facilitate tolbutamide stimulation of immunoreactive insulin release was noted.
Key words
Insulin Sensitivity - Growth Hormone - Tolbutamide
1 This work was supported by Grant No. U-1960 from the Health Research Council of the City of New York.
2 Career Scientist, The Health Research Council of the City of New York.
